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Expression of IL-1 family members upon stimulation with IL-17 differs in keratinocytes derived from psoriasis patients and healthy donors

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Background

A number of studies have challenged the T cell centred pathogenetic view of psoriasis by the finding that epithelium-expressed genes are intimately involved in the inflammatory process. IL-17 is an important inflammatory mediator in skin psoriasis.

Objective

IL-17 is known to act on keratinocytes and we were interested in its impact on expression of pro- and anti-inflammatory IL-1 family members.

Methods

We compared human primary keratinocytes derived from psoriasis patients and healthy individuals using qRT-PCR and ELISA.

Results

In the presence of IL-17 psoriasis derived keratinocytes showed a significantly higher induction of the pro-inflammatory members IL-1F6 and IL-1F9 compared with those from healthy individuals but not of anti-inflammatory members IL-1F5, IL-1F7 or IL-1F3. Both basal, as well as IL-17 induced production of IL-1F2/IL-1β and IL-1F1/IL-1α were found to be significantly lower in psoriasis keratinocytes.

Conclusion

As keratinocytes were derived from epidermal stem cells of the hair follicles and obtained from non-lesional sites, differences found are likely to present an intrinsic feature of psoriasis epithelium. Our data suggest that the significance of IL-1 members as therapeutic targets in psoriasis conditions merits further and thorough investigation.

Author information

Correspondence to M Wittmann.

Additional information

and M Wittmann contributed equally to this work.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Muhr, P., Zeitvogel, J., Werfel, T. et al. Expression of IL-1 family members upon stimulation with IL-17 differs in keratinocytes derived from psoriasis patients and healthy donors. J Transl Med 8, P19 (2010) doi:10.1186/1479-5876-8-S1-P19

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Keywords

  • Psoriasis
  • Healthy Individual
  • Inflammatory Mediator
  • Healthy Donor
  • Hair Follicle