Figure 5

Effects of other lipoxygenase (LOX) inhibitors on ROS generation and apoptosis induced by NSC-741909. Cells were treated with 1 μM NSC-741909 for 6 h (for ROS generation) or 24 h (for analysis of apoptosis and cell viability) in the presence or absence of LOX inhibitors. (A) After treatment, cells were stained with 2', 7'-dichlorofluorescein diacetate, and the fluorescent cell population was counted by flow cytometry and relative ROS production was calculated. (B) Percentage of apoptotic cells determined by flow cytometry. **p < 0.01, compared with cells treated with NSC-741909 alone. (C) Percentage of viable cells determined by the sulforhodamine B assay. Cells treated with solvent (dimethylsulfoxide) alone were used as a control, with viability set at 100%. Each data point represents the mean ± SD of three independent experiments.