Epigenetic change in e-cardherin and COX-2 to predict chronic periodontitis
© Loo et al; licensee BioMed Central Ltd. 2010
Received: 25 June 2010
Accepted: 4 November 2010
Published: 4 November 2010
DNA methylation of certain genes frequently occurs in neoplastic cells. Although the cause remains unknown, many genes have been identified with such atypical methylation in neoplastic cells. The hypermethylation of E-Cadherin and Cyclooxygenase 2 (COX-2) in chronic inflammation such as chronic periodontitis may demonstrate mild lesion/mutation epigenetic level. This study compares the hypermethylation status of E-Cadherin and COX-2 genes which are often found in breast cancer patients with that in chronic periodontitis.
Total DNA was extracted from the blood samples of 108 systemically healthy non-periodontitis subjects, and the gingival tissues and blood samples of 110 chronic periodontitis patient as well as neoplastic tissues of 106 breast cancer patients. Methylation-specific PCR for E-Cadherin and COX-2 was performed on these samples and the PCR products were analyzed on 2% agarose gel.
Hypermethylation of E-Cadherin and COX-2 was observed in 38% and 35% of the breast cancer samples, respectively. In chronic periodontitis patients the detection rate was 25% and 19% respectively, and none was found in the systemically healthy non-periodontitis control subjects. The hypermethylation status was shown to be correlated among the three groups with statistical significance (p < 0.0001). The methylation of CpG islands in E-Cadherin and COX-2 genes in periodontitis patients occurs more frequently in periodontitis patients than in the control subjects, but occurs less frequently than in the breast cancer patients.
This set of data shows that the epigenetic change in E-Cadherin and Cyclooxygenase-2 is associated with chronic periodontitis. The epigenetic changes presented in chronic inflammation patients might demonstrate an irreversible destruction in the tissues or organs similar to the effects of cancer. Chronic periodontitis to some extent might be associated with DNA hypermethylation which is related to cancer risk factors.
DNA methylation is an epigenetic process that alters DNA chemically. It typically occurs in CpG poor regions, and the promoter region of the gene is not methylated . This process is unsurprisingly occurring and is frequently needed for proper development .
Nevertheless, in various types of cancer, including breast ductal carcinoma, abnormal methylation frequently occurs in neoplastic cells. The neoplasia creates a 'methylation imbalance', and causes hypomethylation across the genome and localised hypermethylation within the CpG clusters, or 'islands', in the promoter region of genes that isn't normally methylated . This aberrant methylation in cells causes the silencing of certain genes, including the tumour suppression genes that control DNA repair, cell cycle control, as well as angiogenesis . The cause of such atypical methylation in neoplastic cells is still unknown. CpG island hypermethylation is closely linked to a variety of conditions, including tumorigenesis, chronic inflammation, and intestinal metaplasia .
There are many key indicators of cancer, of which two are significant to this investigation: Cyclooxygenase 2 ( COX-2) and E-Cadherin, as they provide links to chronic periodontitis. COX-2 is, undetectable in most normal tissues, a protein that acts as an enzyme that catalyses the conversion of arachidonic acid into prostaglandins, messengers that promote inflammation [4, 5]. Cancer patients have been shown to have overexpression of COX-2 in their malignant tissues . Research has also shown that people who regularly take non-steroidal anti-inflammatory medication such as COX-2 inhibitors had a lower risk of cancer related illnesses, in particular, colorectal cancer and gastric cancer . E-Cadherin is a transmembrane glycoprotein that is responsible for epithelial intercellular adhesion, and is an important gene that regulates morphogenesis . It is also a tumour suppressing gene. Decreased E-Cadherin expression is characteristic of cancer, including lung, prostate, gastric cancers as well as colorectal carcinoma and breast cancer .
E-Cadherin and COX-2 expression are useful tools in diagnosing and treating cancer. However, they are also indicators of chronic inflammatory diseases such as periodontitis, as both breast cancer and periodontitis are closely related to CpG island hypermethylation resulting in the silencing of these genes.
Periodontitis is a general term for severe infection of the gums causing inflammation. It can be a result of the worsening of gingivitis . The periodontium, the gums and bones that support the teeth, are destroyed, leading to eventual loss of teeth as well as a possible increased risk of heart attacks and stroke [10, 11]. Periodontitis is often chronic and the patient goes through exacerbation and remission periods. It is typically diagnosed via clinical examination, sometimes with the help of x-rays and treatment involves cleaning plaque from the areas under the gums, root scaling and oral antibiotics [12, 13]. In advanced stages of the disease, surgery to remove the deep pockets in the gums can be performed . Periodontal disease was significantly associated with cancer risk .
COX-2 is obviously related to chronic periodontitis as it is the enzyme responsible for controlling the production of prostaglandins that promote the inflammation characteristic of the disease. It is found in the infected gum tissue of gingivitis and periodontitis patients and therefore COX-2 inhibitors improve the symptoms of periodontitis by reducing the swelling and pain . In addition, studies have shown that the expression of E-Cadherin is uniformly present in healthy gums, but decreased expression of the protein were found in the basal areas of the oral gingival epithelium in diseased samples . This suggests that E-Cadherin expression plays a role in the progression of chronic periodontitis.
This study is to determine the relationship between hypermethylation in chronic periodontitis and breast cancer by comparing the hypermethylation of the E-Cadherin and COX-2 genes in the infected gingival tissues of the periodontitis patients and the neoplastic tissues of cancer patients.
Materials and methods
Selection of Control and Patient Samples
Healthy and periodontitis subjects biographical data.
Number of Subjects
Mean Age ± SD
42.8 ± 9.69
42.9 ± 9.71
Mean Pocket Depth (mm)
3.0 ± 0.45
5.5 ± 1.12
17.14 +/- 6.85
61.5 +/- 24.63
Probe with Bleeding
13.77 +/- 6.69
76.49 +/- 19.13
From 2007 to 2009, a total of 110 periodontitis patients were recruited from West China College of Stomatology, Sichuan University. Among these samples, 37 were female and 73 were male, ranging from 18 to 65 years old (median: 44) (Table 1). Their mean pocket depth was 5.5 mm (Table 1). They have been suffering from periodontal disease for over 5 years and have received scaling and root planning every 6 months. Teeth which showed third degree mobility were extracted. Blood was taken from these patients and associated periodontal tissues were cut during tooth extraction for DNA extraction.
Clinical characteristics of 106 breast cancer patients from Sichuan University.
Total number of patients (N) = 106
56.2 (range: 26-85)
≤ 2 cm (T1)
2-5 cm (T2)
>5 cm (T3)
G1 and G2
Written informed consents were obtained from all participants before the procedure which had been approved by ethics committees of the University of Hong Kong and Sichuan University (reference number: 2007SGY028).
Preparation of Control and Patient Blood Samples
Ten milliliters of blood from each patient was collected in lithium heparin tubes (Vacuette, Austria). The blood was centrifuged for 10 minutes at 1500 rpm and plasma was then removed. The cell pellet was transferred to a 50 ml centrifuge tube and red blood cell lysis buffer was added to a final volume of 45 ml. The mixture in the tube was inverted several times and centrifuged for 10 minutes at 1500 rpm. The supernatant was discarded and the cell pellet was washed with 0.9% PBS to be used for DNA extraction.
Sample Preparation and Tissue Collection
DNA from the healthy subjects' blood cells, periodontitis patients' tissues and breast cancer tumour samples were extracted by Geneaid® DNA Mini Kit (Tissue) (Geneaid, Taiwan). The provided Micropestle was used to grind the tissue to a pulp. 200 μl of GT Buffer was added into the tube and the sample tissue was continually homogenized with grinding. 20 μl of Proteinase K (10 mg/ml) was added to the sample mixture and mixed by vortexing. The sample was lysed by incubation at 60°C for 30 minutes, and the tube was inverted every 5 minutes. At this time, the required Elution Buffer was preheated at 70°C. 200 μl of ethanol was added to the sample lysate and mixed immediately by vortexing for 10 seconds. Pipetting was performed to break up the precipitate formed. A GD Column was placed in a 2 ml Collection Tube. All the mixture from previous step (including any precipitate) was applied to the GD column, and was centrifuged at 13,000 rpm for 2 minutes. The Collection Tube containing the flow-through was discarded and the GD column was transferred in a new 2 ml Collection Tube. 500 μl of Wash Buffer (ethanol added) was added into the column and was centrifuged at 13,000 rpm for 30 seconds. The flow-through was discarded and the GD column was placed back into the Collection Tube. The wash step by adding Wash Buffer was performed once again. The flow-through was, again, discarded and the GD Column was placed back to the Collection Tube and was centrifuged at full speed for 3 minutes to dry the column matrix. The dry GD Column was transferred in a clean 1.5 ml microcentrifuge tube. 100 μl of preheated Elution Buffer was added into the centre of the column matrix and was stood for 5 minutes until the Elution Buffer was absorbed by the matrix. 13,000 rpm centrifugation was performed for 30 seconds to elute purified DNA.
Primers used and their corresponding sequences
The relative risk of hypermethylation status of E-Cadherin and COX-2 among cancer patients, periodontitis patients and healthy subjects was analysed. Chi-square test was performed to analyse the distribution of hypermethylation in test groups compared with control using SPSS 12.0 (SPSS Inc., USA).
Status of E-Cadherin and COX-2 hypermethylation in healthy, periodontitis patients and breast cancer patients.
Hypermethylation of Cox-2(%)
Relative Risk (95% confidence Intervals)
Hypermethylation of E-cadherin(%)
Relative Risk (95% confidence Intervals)
(N = 108)
(N = 110)
p < 0.0001
p < 0.0001
Breast cancer patients
(N = 106)
p < 0.0001
p < 0.0001
100 bp DNA marker was selected as a reference. The products of the PCR generated by methylation-specific PCR were used in electrophoresis, using a 100 bp ladder (Invitrogen, USA) and the images were captured under UV light. The hypermethylation status of E-Cadherin and COX-2 was shown to be correlated between the three groups with statistical significance (p < 0.0001).
There are a number of other widely accepted factors contributing to a patient's relative risk to periodontitis. These include age, gender, oral hygiene, smoking, poor glycemic control in diabetic patients, genetics, systemic diseases, etc [16–22]. Apart from these factors, there is also the epigenetic factor. However, the impact of epigenetics on periodontitis and other chronic inflammatory diseases is not studied in as much depth as the other host and genetic factors. Some research has been done on aberrant CpG hypermethylation in other chronic inflammatory diseases such as gastritis and ulcerative colitis but next to none has been done on periodontitis [23, 3].
Currently, the diagnosing of chronic periodontitis relies on clinical inspection via probing depths, attachment levels, bleeding, plaque index and the use of x-rays or other radiographic methods . The oral cavity of the patient is usually initially inspected for the disease status. Casts, photos and often X-rays are collected for interpretation. However, diagnoses of the disease by monitoring epigenetic changes such as E-Cadherin and COX-2 expression are very rarely used. Although only E-Cadherin and COX-2 were studied in this investigation, a wide variety of other epigenetic changes may be factors involved in the progression of chronic inflammation diseases like periodontitis, and these could prove to be valuable tools in the diagnosis of such diseases. Furthermore, the potential applications of these changes are not limited to diagnostic purposes. Studying epigenetic changes and their relationships with chronic inflammation may provide not only new diagnostic methods, but it could also be useful in developing new treatments.
Periodontal pathogens may induce chronic inflammation and inflammatory responses. These responses may promote carcinogenesis and disrupt the cell cycle . Both genetic and other factors, such as environmental, epigenetic factors may cause chronic inflammatory diseases . A good example is the different genotypes caused by single nucleotide polymorphisms (SNPs) of the pro-inflammatory genes , such as interleukin-1 (IL-1), IL-6, and neutrophil. It seems that genetic factors of the host may decide which bacteria to colonize the host, different gene polymorphisms increase the growth of specific bacteria .
Epigenetics may be related to tumourigenesis , and other diseases such as cardiovascular diseases. A positive link between chronic inflammation and cancer has been published although the progression mechanism is still debated. Increased DNA methylation has been found in chronic inflammation such as chronic gastritis , ulcerative colitis  as well as in prostate cancers .
IL-6 is produced at the inflammation site, which regulates the transition of neutrophils to macrophages, and it helps the stimulation of T and B cells. Its high level has been found in different infections and cancers. Treatment which targets IL-6 and its signalling may prevent chronic inflammatory diseases . DNA methyltransferase (DNMT1) maintains the methylation pattern, when the IL-6 level is low, the p53 promoter region is modified by DNMT-1 and thus p53 expression decreases. The disrupted expression of this tumour suppressor gene plays a key role in cancer initiation . Although the effect of IL-6 to cancer is still unknown, this cytokine may provide a link from bacterial infection to inflammation and cancer. Changes and damages in cells and tissues during inflammation may initiate cancer development. Specific markers of inflammation can be studied to look at the association with increased methylation, so that the underlying mechanisms between chronic inflammation and cancer can be revealed.
The results of this experiment show that methylation of CpG islands in the E-Cadherin and COX-2 genes in periodontitis patients occurs more frequently than in the healthy control group, but less frequently than in the breast cancer patients as supported by Pearson chi-square test (Table 4). The difference in the percentage frequency of hypermethylation for both genes between the healthy subjects and chronic periodontitis patients is statistically significant (p < 0.0001). The finding showed that chronic inflammation and cancer may share the same pattern of genomic and epigenetic changes. Methylation contributes to chronic inflammation, periodontal disease might be a marker of a susceptible immune system or might directly affect cancer risk and it may occur through possible biologic mechanisms [14, 35]. The biological pathways may involve epigenetic changes, this altered CpG region presented in chronic inflammation patients, demonstrates an irreversible destruction in the tissues or organs similar to the cancer effects.
These data confirms that E-Cadherin and COX-2 expression are factors related to periodontitis. Further study of similar epigenetic changes may prove to be extremely useful in the diagnosis and treatment of chronic periodontitis in the future.
This study was supported by Scientific Support Project of Science and Technology Department of Sichuan province, China (2007SGY028).
- Bock C, Paulsen M, Tierling S, Mikeska T, Lengauer T, Walter J: CpG island methylation in human lymphocytes is highly correlated with DNA sequence, repeats, and predicted DNA Structure. PLoS Genetics. 2006, 2: e26-10.1371/journal.pgen.0020026.PubMedPubMed CentralView ArticleGoogle Scholar
- Lui E, Loo W, Zhu L, Cheung M, Chow L: DNA hypermethylation of TIMP3 gene in invasive breast ductal carcinoma. Biomed & Pharmacother. 2005, 59: S363-S365. 10.1016/S0753-3322(05)80079-4.View ArticleGoogle Scholar
- Kang G, Lee H, Hwang K, Lee S, Kim J, Kim J: Abberant CpG island hypermethylation of chronic gastritis, in relation to aging, gender, intestinal metaplasia and chronic inflammation. Am J Pathol. 2003, 163: 1551-6.PubMedPubMed CentralView ArticleGoogle Scholar
- Sminia P, Kuipers G, Geldof A, Lafleur V, Slotman B: COX-2 inhibitors act as radiosensitizer in tumour treatment. Biomed & pharmacother. 2005, 59: 272-275. 10.1016/S0753-3322(05)80044-7.View ArticleGoogle Scholar
- Chow L, Zhu L, Loo W, Lui E: Aberrant methylation of cyclooxygenase-2 in breast cancer patients. Biomed Pharmacother. 2005, 59: S264-67. 10.1016/S0753-3322(05)80042-3.PubMedView ArticleGoogle Scholar
- Wu Y, Fu S, Zhang Y, Qiao M, Chen Y: Cyclooxygenase-2 inhibitors suppress angiogenesis and growth of gastric cancer xenografts. Biomed & pharmacother. 2005, 59: S289-92. 10.1016/S0753-3322(05)80048-4.View ArticleGoogle Scholar
- Tycko B: Epigenetic gene silencing in cancer. J Clin Invest. 2000, 105: 401-407. 10.1172/JCI9462.PubMedPubMed CentralView ArticleGoogle Scholar
- Kowalski P, Rubin M, Kleer C: E-Cadherin expression in primary carcinomas of the breast and its distant metastases. Breast Cancer Res. 2003, 5: R217-R222. 10.1186/bcr651.PubMedPubMed CentralView ArticleGoogle Scholar
- Nagarakanti S: Differential expression of E-Cadherin and cytokeratin 19 and net proliferative rate of gingival keratinocytes in oral epithelium in periodontal health and disease. J Periodontol. 2007, 78: 2197-202. 10.1902/jop.2007.070070.PubMedView ArticleGoogle Scholar
- Irwin C, Mullally B, Ziada H, Byrne P, Allen E: Periodontics: 9. Periodontitis and systemic conditions--is there a link?. Dent Update. 2008, 35: 92-101.PubMedGoogle Scholar
- Nakajima T, Honda T, Domon H, Okui T, Kajita K, Ito H, Takahashi N, Maekawa T, Tabeta K, Yamazaki K: Periodontitis-associated up-regulation of systemic inflammatory mediator level may increase the risk of coronary heart disease. J Periodontal Res. 2010, 45: 116-22. 10.1111/j.1600-0765.2009.01209.x.PubMedView ArticleGoogle Scholar
- Pihlstrom B: Periodontal risk assessment, diagnosis and treatment planning. Periodontol 2000. 2001, 25: 37-58. 10.1034/j.1600-0757.2001.22250104.x.PubMedView ArticleGoogle Scholar
- Watts T: Periodontitis for medical practitioners. Br Med J. 1998, 316: 993-6.View ArticleGoogle Scholar
- Michaud DS, Liu Y, Meyer M, Giovannucci E, Joshipura K: Periodontal disease, tooth loss, and cancer risk in male health professionals: a prospective cohort study. Lancet Oncol. 2008, 9: 550-8. 10.1016/S1470-2045(08)70106-2.PubMedPubMed CentralView ArticleGoogle Scholar
- Pinho M, Pereira L, de Souza S, Palioto D, Grisi M, Novaes A, Taba M: Short-term effect of COX-2 selective inhibitor as an adjunct for the treatment of periodontal disease: a clinical double-blind study in humans. Braz Dent J. 2008, 19: 323-8. 10.1590/S0103-64402008000400007.View ArticleGoogle Scholar
- Torres de Heens G, Van der Velden U, Loos B: Cigarette smoking enhances T cell activation and Th2 immune response; and aspect of the pathophysiology in periodontal disease. Cytokine. 2009, 47: 157-61. 10.1016/j.cyto.2009.05.006.View ArticleGoogle Scholar
- Van Dyke T, Dave S: Risk factors for periodontitis. J Int Acad Periodontol. 2005, 7: 3-7.PubMedPubMed CentralGoogle Scholar
- Rosania A, Low K, McCormick C, Rosania D: Stress, depression, cortisol, and periodontal disease. J Periodontol. 2009, 80: 260-6. 10.1902/jop.2009.080334.PubMedView ArticleGoogle Scholar
- Desvarieux M, Schwahn C, Völzke H, Demmer R, Lüdemann J, Kessler C, Jacobs D, John U, Kocher T: Gender differences in the relationship between periodontal disease, tooth loss, and atherosclerosis. Stroke. 2004, 35: 2029-35. 10.1161/01.STR.0000136767.71518.36.PubMedView ArticleGoogle Scholar
- Timmerman M, Van der Weijden G: Risk factors for periodontitis. Int J Dent Hyg. 2006, 4: 2-7. 10.1111/j.1601-5037.2006.00168.x.PubMedView ArticleGoogle Scholar
- Van der Stelt P: Modern radiographic methods in the diagnosis of periodontal disease. Adv Dent Res. 1993, 7: 158-62.PubMedGoogle Scholar
- Reichert S, Stein J, Gautsch A, Schaller H, Machulla H: Gender Differences in HLA phenotype frequencies found in German patients with generalized aggressive periodontitis and chronic periodontitis. Oral Microbiol Immunol. 2002, 17: 360-8. 10.1034/j.1399-302X.2002.170605.x.PubMedView ArticleGoogle Scholar
- Issa J, Ahuja N, Toyota M, Bronner M, Brentnall T: Accelerated age-related CpG island methylation in ulcerative colitis. Cancer Res. 2001, 61: 3573-7.PubMedGoogle Scholar
- Armitage G, Robertson P: The biology, prevention, diagnosis and treatment of periodontal diseases: scientific advances in the United States. J Am Dent Assoc. 2009, 140: 36S-43S.PubMedView ArticleGoogle Scholar
- Coussens LM, Werb Z: Inflammation and cancer. Nature. 2002, 420: 860-867. 10.1038/nature01322.PubMedPubMed CentralView ArticleGoogle Scholar
- Michalowicz BS, Aeppli D, Virag JG, Klump DG, Hinrichs JE, Segal NL, Bouchard TJ, Pihlstom BL: Periodontal findings in adult twins. J Peridontal. 1991, 62: 293-299.View ArticleGoogle Scholar
- McLean Mairi, El-Omar Emad: Genetic aspects of inflammation. Current Opinion in Pharmacology. 2009, 9: 370-374. 10.1016/j.coph.2009.06.003.PubMedView ArticleGoogle Scholar
- Nibali Luigi, Donos Nikos, Henderson Brian: Peridontal infectogenomics. Journal of Medical Microbiology. 2009, 58: 1269-1274. 10.1099/jmm.0.012021-0.PubMedView ArticleGoogle Scholar
- Nephew KP, Huang TH: Epigenetic gene silencing in cancer initiation and progression. Cancer Lett. 2003, 190: 125-33. 10.1016/S0304-3835(02)00511-6.PubMedView ArticleGoogle Scholar
- Kang GH, Lee HJ, Hwang KS, Lee S, Kim JH, Kim JS: Aberrant CpG island hypermethylation of chronic gastritis, in relation to aging, gender, intestinal metaplasia, and chronic inflammation. Am J Pathol. 2003, 163: 15516-Google Scholar
- Issa Jean-Pierre, Ahuja Nita, Toyota Minoru, Bronner Mary, Brentnall Teresa: Accelerated Age-related CpG Island Methylation in Ulcerative Colitis. Cancer Res. 2001, 63: 3573-3577.Google Scholar
- Lee WH, Morton RA, Epstein JI: Cytidine methylation of regulatory sequences near the π-class glutathione S-transferase gene accompanies human prostatic carcinogenesis. Proc Natl Acad Sci USA. 1994, 91: 11733-7. 10.1073/pnas.91.24.11733.PubMedPubMed CentralView ArticleGoogle Scholar
- Gabay Cem: Interleukin-6 and chronic inflammation. Arthritis Research & Therapy. 2006, 8 (Suppl 2): S3-10.1186/ar1917.View ArticleGoogle Scholar
- Hodge David, Peng Benjamin, Cherry James, Hurt Elaine, Fox Stephen, Kelley James, Munroe David, William L: Farrar. Interleukin 6 Supports the Maintenance of p53 Tumour Suppressor Gene Promoter Methylation. Cancer Res. 2005, 65: 4673-4682. 10.1158/0008-5472.CAN-04-3589.PubMedView ArticleGoogle Scholar
- Michaud DS, Joshipura K, Giovannucci E, Fuchs CS: A prospective study of periodontal disease and pancreatic cancer in US male health professionals. J Natl Cancer Inst. 2007, 99: 171-5. 10.1093/jnci/djk021.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.