- Open Access
Circulating endothelial progenitor cells: a new approach to anti-aging medicine?
Journal of Translational Medicine volume 7, Article number: 106 (2009)
Endothelial dysfunction is associated with major causes of morbidity and mortality, as well as numerous age-related conditions. The possibility of preserving or even rejuvenating endothelial function offers a potent means of preventing/treating some of the most fearful aspects of aging such as loss of mental, cardiovascular, and sexual function.
Endothelial precursor cells (EPC) provide a continual source of replenishment for damaged or senescent blood vessels. In this review we discuss the biological relevance of circulating EPC in a variety of pathologies in order to build the case that these cells act as an endogenous mechanism of regeneration. Factors controlling EPC mobilization, migration, and function, as well as therapeutic interventions based on mobilization of EPC will be reviewed. We conclude by discussing several clinically-relevant approaches to EPC mobilization and provide preliminary data on a food supplement, Stem-Kine, which enhanced EPC mobilization in human subjects.
The endothelium plays several functions essential for life, including: a) acting as an anticoagulated barrier between the blood stream and interior of the blood vessels; b) allowing for selective transmigration of cells into and out of the blood stream; c) regulating blood flow through controlling smooth muscle contraction/relaxation; and d) participating in tissue remodeling . A key hallmark of the aging process and perhaps one of the causative factors of health decline associated with aging appears to be loss of endothelial function. Whether as a result of oxidative stress, inflammatory stress, or senescence, deficiencies in the ability of the endothelium to respond to physiological cues can alter mental , sexual , visual , and respiratory  ability. Specifically, minute alterations in the ability of endothelium to respond to neurotransmitter induced nitric oxide causes profound inability to perform even simple mental functions [6, 7]. Small increases in angiogenesis in the retina as a result of injury or glucose are associated with wet macular degeneration blindness . Atherosclerosis of the penile vasculature is a major cause of erectile dysfunction . The pulmonary endothelium's sensitivity to insult can cause hypertension and associated progression to decreased oxygen delivery .
Health of the endothelium can be quantified using several methods, including assessment of the physical and mechanical features of the vessel wall, assaying for production of systemic biomarkers released by the endothelium, and quantification of ability of blood vessels to dilate in response to increased flow . Of these, one of the most commonly used assays for endothelium function is the flow mediated dilation (FMD) assay. This procedure usually involves high resolution ultrasound assessment of the diameter of the superficial femoral and brachial arteries in response to reactive hyperemia induced by a cuff. The extent of dilatation response induced by the restoration of flow is compared to dilatation induced by sublingual glyceryl trinitrate. Since the dilatation induced by flow is dependent on the endothelium acting as a mechanotransducer and the dilatation induced by glyceryl trinitrate is based on smooth muscle responses, the difference in dilatation response serves as a means of quantifying one aspect of endothelial health [12, 13]. This assay has been used to show endothelial dysfunction in conditions such as healthy aging [14–16], as well as various diverse inflammatory states including renal failure , rheumatoid arthritis , Crohn's Disease , diabetes , heart failure , and Alzheimer's . Although it is not clear whether reduction in FMD score is causative or an effect of other properties of endothelial dysfunction, it has been associated with: a) increased tendency towards thrombosis, in part by increased von Willibrand Factor (vWF) levels , b) abnormal responses to injury, such as neointimal proliferation and subsequent atherosclerosis , and c) increased proclivity towards inflammation by basal upregulation of leukocyte adhesion molecules .
As part of age and disease associated endothelial dysfunction is the reduced ability of the host to generate new blood vessel . This is believed to be due, at least in part, to reduction of ischemia inducible elements such as the HIF-1 alpha transcription factor which through induction of stromal derived factor (SDF-1) and vascular endothelial growth factor (VEGF) secretion play a critical role in ability of endothelium to migrate and form new capillaries in ischemic tissues [27, 28]. Accordingly, if one were to understand the causes of endothelial dysfunction and develop methods of inhibiting these causes or stimulating regeneration of the endothelium, then progression of many diseases, as well as possible increase in healthy longevity may be achieved.
Endothelial Progenitor Cells: Rejuvenators of the Vasculature
During development endothelial cells are believed to originate from a precursor cell, the hemangioblast, which is capable of giving rise to both hematopoietic and endothelial cells . Classically the endothelium was viewed as a fixed structure with relatively little self renewal, however in the last two decades this concept has fundamentally been altered. The current hypothesis is that the endothelium is constantly undergoing self renewal, especially in response to stress. A key component of endothelial turnover appears to be the existence of circulating endothelial progenitor (EPC) cells that appear to be involved in repair and angiogenesis of ischemic tissues. An early study in 1963 hinted at the existence of such circulating EPC after observations of endothelial-like cells, that were non-thrombogenic and morphologically appeared similar to endothelium, were observed covering a Dacron graft that was tethered to the thoracic artery of a pig . The molecular characterization of the EPC is usually credited to a 1997 paper by Asahara et al. in which human bone marrow derived VEGR-2 positive, CD34 positive monocyte-like cells were described as having ability to differentiate into endothelial cells in vitro and in vivo based on expression of CD31, eNOS, and E-selectin . These studies were expanded into hindlimb ischemia in mouse and rabbit models in which increased circulation of EPC in response to ischemic insult was observed . Furthermore, these studies demonstrated that cytokine-induced augmentation of EPC mobilization elicited a therapeutic angiogenic response. Using irradiated chimeric systems, it was demonstrated that ischemia-mobilized EPC derive from the bone marrow, and that these cells participate both in sprouting of pre-existing blood vessels as well as the initiation of de novo blood vessel production . Subsequent to the initial phenotypic characterization by Asahara et al , more detailed descriptions of the human EPC were reported. For example, CD34 cells expressing the markers VEGF-receptor 2, CD133, and CXCR-4 receptor, with migrational ability to VEGF and SDF-1 has been a more refined EPC definition . However there is still some controversy as to the precise phenotype of the EPC, since the term implies only ability to differentiate into endothelium. For example, both CD34+, VEGFR2+, CD133+, as well as CD34+, VEGFR2+, CD133- have been reported to act as EPC . More recent studies suggest that the subpopulation lacking CD133 and CD45 are precursor EPC . Other phenotypes have been ascribed to cells with EPC activity, one study demonstrated monocyte-like cells that expressing CD14, Mac-1 and the dendritic cell marker CD11c have EPC activity based on uptake of acetylated LDL and binding to the ulex-lectin [37, 38].
While the initial investigations into the biology of EPC focused around acute ischemia, it appears that in chronic conditions circulating EPC may play a role in endothelial turnover. Apolipoprotein E knockout (ApoE KO) mice are genetically predisposed to development of atherosclerosis due to inability to impaired catabolism of triglyceride-rich lipoproteins. When these mice are lethally irradiated and reconstituted with labeled bone marrow stem cells, it was found that areas of the vasculature with high endothelial turnover, which were the areas of elevated levels of sheer stress, had incorporated the majority of new endothelial cells derived from the bone marrow EPC . The possibility that endogenous bone marrow derived EPC possess such a regenerative function was also tested in a therapeutic setting. Atherosclerosis is believed to initiate from endothelial injury with a proliferative neointimal response that leads to formation of plaques. When bone marrow derived EPC are administered subsequent to wire injury, a substantial reduction in neointima formation was observed . The argument can obviously made that wire injury of an artery does not resemble the physiological conditions associated with plaque development. To address this, Wassmann et al , used ApoE KO mice that were fed a high cholesterol diet and observed reduction in endothelial function as assessed by the flow mediated dilation assay. When EPC were administered from wild-type mice restoration of endothelial responsiveness was observed.
In the context of aging, Edelman's group performed a series of interesting experiments in which 3 month old syngeneic cardiac grafts were heterotopically implanted into 18 month old recipients. Loss of graft viability, associated with poor neovascularization, was observed subsequent to transplanting, as well as subsequent to administration of 18 month old bone marrow mononuclear cells. In contrast, when 3 month old bone marrow mononuclear cells were implanted, grafts survived. Antibody depletion experiments demonstrated bone marrow derived platelet derived growth factor (PDGF)-BB was essential in integration of the young heart cells with the old recipient vasculature . These experiments suggest that young EPC or EPC-like cells have ability to integrate and interact with older vasculature. What would be interesting is to determine whether EPC could be "revitalized" ex vivo by culture conditions or transfection with therapeutic genes such as PDGF-BB.
Given animal studies suggest EPC are capable of replenishing the vasculature, and defined markers of human EPC exist, it may be possible to contemplate EPC-based therapies. Two overarching therapeutic approaches would involve utilization of exogenous EPC or mobilization of endogenous cells. Before discussing potential therapeutic interventions, we will first examine several clinical conditions in which increasing circulating EPC may play a role in response to injury.
Clinical Increase of Circulating EPC as a Response to Injury
Tissue injury and hypoxia are known to generate chemoattractants that potentially are responsible for mobilization of EPC. Reduction in oxygen tension occurs as a result of numerous injuries including stroke, infarction, or contusion. Oxygen tension is biologically detected by the transcription factor HIF-1 alpha, which upon derepression undergoes nuclear translocation. This event causes upregulated expression of a plethora of angiogenesis promoting cytokines and chemoattractants , such as stromal derived factor (SDF)-1 and VEGF [44, 45]. On the other hand, tissue necrosis causes release of "danger signals" such as HMBG1, a nuclear factor that has direct chemoattractant activity on mesoangioblasts, a type of EPC [46, 47]. It has been demonstrated that this systemic release of chemoattractant cytokines after vascular injury or infarct is associated with mobilization of endogenous bone marrow cells and EPC .
Myocardial infarction has been widely studied in the area of regenerative medicine in which cellular and molecular aspects of host response post-injury are relatively well defined. EPC mobilization after acute ischemia has been demonstrated in several cardiac infarct studies. This was first reported by Shintani et al who observed increased numbers of CD34 positive cells in 16 post infarct patients on day 7 as compared to controls. The rise in CD34 cells correlated with ability to differentiate into cells morphologically resembling endothelium and expressing endothelial markers KDR and CD31. Supporting the concept that response to injury stimulates EPC mobilization, a rise in systemic VEGF levels was correlated with increased EPC numbers . A subsequent study demonstrated a similar rise in circulating EPC post infarct. Blood was drawn from 56 patients having a recent infarct (<12 hours), 39 patients with stable angina, and 20 healthy controls. Elevated levels of cells expressing CD34/CXCR4+ and CD34/CD117+ and c-met+ were observed only in the infarct patients which were highest at the first blood draw. In this study the mobilized cells not only expressed endothelial markers, but also myocytic and cardiac genes . The increase in circulating EPC at early timepoints post infarction has been observed by other groups, and correlated with elevations in systemic VEGF and SDF-1 [50, 51].
In the case of cerebral infarction studies support the concept that not only are EPC mobilized in response to ischemia, but also that the extent of mobilization may be associated with recovery. In a trial of 48 patients suffering primary ischemic stroke, mobilization of EPC was observed in the first week in comparison to control patients. EPC were defined as cells capable of producing endothelial colony forming units. A correlation between improved outcome at 3 months and extend of EPC mobilization was observed based on the NIHSS and Rankin score . In a similar study, Dunac et al reported on circulating CD34 levels of 25 patients with acute stroke for 14 days. A correlation between improvement on the Rankin scale and increased circulating CD34 cells was reported . Noteworthy was that the level of CD34 mobilization was similar to that observed in patients treated with the mobilize G-CSF. In a larger study, Yip et al examined EPC levels in 138 consecutive patients with acute stroke and compared them to 20 healthy volunteers and in 40 at-risk control subjects . Three EPC phenotypes were assessed by flow cytometry at 48 hours after stroke: a) CD31/CD34, b) CD62E/CD34, and c) KDR/CD34. Diminished levels of all three EPC subsets in circulation was predictive of severe neurological impairment NIHSS >/= 12, while suppressed levels of circulating CD31/34 cells was correlated with combined major adverse clinical outcomes as defined by recurrent stroke, any cause of death, or NIHSS >/= 12. Increased levels of the KDR/CD34 phenotype cells was strongly associated with NIHSS > or = 4 on day 21. Although these studies do not directly demonstrate a therapeutic effect of the mobilized EPC, animal studies in the middle cerebral artery ligation stroke model have demonstrated positive effects subsequent to EPC administration [55, 56], an effect which appears to be at least partially dependent on VEGF production from the EPC .
Another ischemia-associated tissue insult is acute respiratory distress syndrome (ARDS), in which respiratory failure often occurs as a result of disruption of the alveolar-capillary membrane, which causes accumulation of proteinaceous pulmonary edema fluid and lack of oxygen uptake ability . In this condition there has been some speculation that circulating EPC may be capable of restoring injured lung endothelium. For example, it is known that significant chimerism (37-42%) of pulmonary endothelial cells occurs in female recipients of male bone marrow transplants . Furthermore, in patients with pneumonia infection there is a correlation between infection and circulating EPC, with higher numbers of EPC being indicative of reduced fibrosis . The possibility that EPC are mobilized during ARDS and may be associated with benefit was examined in a study of 45 patients with acute lung injury in which a correlation between patients having higher number of cells capable of forming endothelial colonies in vitro and survival was made. Specifically, the patients with a colony count of >or= 35 had a mortality of approximately 30%, compared to patients with less than 35 colonies, which had a mortality of 61%. The correlation was significant after multivariable analysis correcting for age, sex, and severity of illness . From an interventional perspective, transplantation of EPC into a rabbit model of acute lung injury resulted in reduction of leukocytic infiltrates and preservation of pulmonary cellular integrity .
Sepsis is a major cause of ARDS and is associated with acute systemic inflammation and vascular damage. Septic patients have elevated levels of injury associated signals and EPC mobilizers such as HMGB1 , SDF-1 , and VEGF . Significant pathology of sepsis is associated with vascular leak and disseminated intravascular coagulation . The importance of the vasculature in sepsis can perhaps be supported by the finding that the only drug to have an impact on survival, Activated Protein C, acts primarily through endothelial protection . Septic patients are known to have increased circulating EPC as compared to controls. Becchi et al observed a correlation between VEGF and SDF-1 levels with a 4-fold rise in circulating EPC in septic patients as compared to healthy controls . A correlation between EPC levels and survival after sepsis was reported in a study of 32 septic patients, 15 ICU patients, and 15 controls. Of the 8 patients who succumbed to sepsis by 28 days, as compared to 24 survivors, a significantly reduced EPC number in non-survivors was reported .
It appears that in conditions of acute injury, elevation of EPC in circulation occurs. Although studies in stroke [52–54], ARDS , and sepsis  seem to correlate outcome with extend of mobilization, work remains to be performed in assessing whether it is the EPC component that is responsible for benefits or other confounding variables. Taking into account the possibility that EPC may act as an endogenous repair mechanism, we will discuss data in chronic degenerative conditions in which circulating EPC appear to be suppressed.
Chronic Inflammatory Disease Inhibit Circulating EPC
There is need for angiogenesis and tissue remodeling in the context of various chronic inflammatory conditions. However in many situations it is the aberrant reparative processes that actually contribute to the pathology of disease. Examples of this include: the process of neointimal hyperplasia and subsequent plaque formation in response to injury to the vascular wall , the process of hepatic fibrosis as opposed to functional regeneration , or the post-infarct pathological remodeling of the myocardium which results in progressive heart failure . In all of these situations it appears that not only the lack of regenerative cells, but also the lack of EPC is present. Conceptually, the need for reparative cells to heal the ongoing damage may have been so overwhelming that it leads to exhaustion of EPC numbers and eventual reduction in protective effect. Supporting this concept are observations of lower number of circulating EPC in inflammatory diseases, which may be the result of exhaustion. Additionally, the reduced telomeric length of EPC in patients with coronary artery disease , as well as reduction of telomere length in the EPC precursors that are found in the bone marrow [73, 74] suggests that exhaustion in response to long-term demand may be occurring. If the reparatory demands of the injury indeed lead to depletion of EPC progenitors, then administration of progenitors should have therapeutic effects.
Several experiments have shown that administration of EPC have beneficial effects in the disease process. For example, EPC administration has been shown to: decrease balloon injury induced neointimal hyperplasia , b) suppress carbon tetrachloride induced hepatic fibrosis [76, 77], and inhibit post cardiac infarct remodeling . One caveat of these studies is that definition of EPC was variable, or in some cases a confounding effect of coadministered cells with regenerative potential may be present. However, overall, there does appear to be an indication that EPC play a beneficial role in supporting tissue regeneration. As discussed below, many degenerative conditions, including healthy aging, are associated with a low-grade inflammation. There appears to be a causative link between this inflammation and reduction in EPC function.
Inflammatory conditions present with features, which although not the rule, appear to have commonalities. For example, increases in inflammatory markers such as C-reactive protein (CRP), erythrocyte sedimentation rate, and cytokines such as TNF-alpha and IL-18 have been described in diverse conditions ranging from organ degenerative conditions such as heart failure [79, 80], kidney failure [81, 82], and liver failure [83, 84] to autoimmune conditions such as rheumatoid arthritis  and Crohn's Disease , to healthy aging [87, 88]. Other markers of inflammation include products of immune cells such as neopterin, a metabolite that increases systemically with healthy aging , and its concentration positively correlates with cognitive deterioration in various age-related conditions such as Alzheimer's . Neopterin is largely secreted by macrophages, which also produce inflammatory mediators such as TNF-alpha, IL-1, and IL-6, all of which are associated with chronic inflammation of aging . Interestingly, these cytokines are known to upregulate CRP, which also is associated with aging . While there is no direct evidence that inflammatory markers actively cause shorted lifespan in humans, strong indirect evidence of their detrimental activities exists. For example, direct injection of recombinant CRP in healthy volunteers induces atherothrombotic endothelial changes, similar to those observed in aging . In vitro administration of CRP to endothelial cells decreases responsiveness to vasoactive factors, resembling the human age-associated condition of endothelial hyporesponsiveness .
Another important inflammatory mediator found elevated in numerous degenerative conditions is the cytokine TNF-alpha. Made by numerous cells, but primarily macrophages, TNF-alpha is known to inhibit proliferation of repair cells in the body, such as oligodendrocytes in the brain , and suppress activity of endogenous stem cell pools [96, 97]. TNF-alpha decreases EPC viability, an effect that can be overcome, at least in part by antioxidant treatment . Administration of TNF-alpha blocking agents has been demonstrated to restore both circulating EPC, as well as endothelial function in patients with inflammatory diseases such as rheumatoid arthritis [18, 99, 100],
It appears that numerous degenerative conditions are associated with production of inflammatory mediators, which directly suppress EPC production or activity. This may be one of the reasons for findings of reduced EPC and FMD indices in patients with diverse inflammatory conditions. In addition to the direct effects, the increased demand for de novo EPC production in inflammatory conditions would theoretically lead to exhaustion of EPC precursors cells by virtue of telomere shortening.
EPC Exhaustion as a Mechanism of Chronic Inflammation
On average somatic cells can divide approximately 50 times, after which they undergo senescence, die or become cancerous. This limited proliferative ability is dependent on the telomere shortening problem. Every time cells divide the ends of the chromosomes called "telomeres" (complexes of tandem TTAGGGG repeats of DNA and proteins), are not completely replicated, thus they progressively get shorter . Once telomeres reach a critical limit p53, p21, and p16 pathways are activated as a DNA damage response reaction instructing the cell to exit cell cycling. Associated with the process of senescence, the cells start expressing inflammatory cytokines such as IL-1 [102, 103], upregulation of adhesion molecules that attract inflammatory cells such as monocytes [104, 105], and morphologically take a flattened, elongated appearance. Physiologically, the process of cellular senescence caused in response to telomere shortening is believed to be a type of protective mechanism that cells have to prevented carcinogenesis . At a whole organism level the association between telomere length and age has been made , as well, disorders of premature aging such as ataxia telangiectasia are characterized by accelerated telomere shortening .
The importance of this limited proliferative ability becomes apparent in our discussion of EPC. In general there is a need for continual endothelial cell replacement from EPC. Because the endothelial cells are exposed to enormous continual sheer stress of blood flow, mechanisms of repair and proliferation after injury need to exist. Theoretically, the more sheer stress on a particular artery, the more cell division would be required to compensate for cell loss. Indeed this appears to be the case. For example, telomeres are shorter in arteries associated with higher blood flow and sheer stress (like the iliac artery) as compared to arteries of lower stress such as the mammary artery . The theory that senescence may be associated with atherosclerosis is supported since the iliac artery, which is associated with higher proliferation of endothelial cells and is also at a higher risk of atherosclerosis, thus prompting some investigators to propose atherosclerosis being associated with endothelial senescence [110, 111].
In an interesting intervention study Satoh et al examined 100 patients with coronary artery disease and 25 control patients. Telomere lengths were reduced in EPC of coronary artery disease patients as compared to controls. Lipid lowering therapy using agents such as atorvastatin has previously been shown to reduced oxidative stress and increase circulating EPC. Therapy with lipid lowering agents in this study resulted in preservation of telomeric length, presumably by decreasing the amount of de novo EPC produced, as well as oxidative stress leading to telomere erosion . One important consideration when discussing telomere shortening of EPC is the difference between replicative senescence, which results from high need for differentiated endothelial cells, and stress induced senescence, in which inflammatory mediators can directly lead to telomere shortening. For example, smoking associated oxidative stress has been linked to stress induced senescence in clinical studies , whereas other studies have implicated inflammatory agents such as interferon gamma , TNF-alpha , and oxidative mediators as inducers of stress induced senescence .
Intervening to Increase Vascular Health and EPC
Based on the above descriptions, it appears that in degenerative conditions, as well as in aging, an underlying inflammatory response occurs that is directly or indirectly associated with inhibition of circulating EPC activity. Directly, inflammation is known to suppress stem cell turnover and activity of EPC. Indirectly, inflammatory conditions place increased demands on the EPC progenitors due to overall increased need for EPC. Accordingly, an intervention strategy may be reduction in inflammatory states: this may be performed in a potent means by administration of agents such as TNF blockers , or more chronically by dietary supplements [117, 118], caloric restriction , exercise [120, 121], consuming blueberries , green tea , or statin therapy . One example of a large scale intervention was the JUPITER trial of >17,000 healthy persons without hyperlipidemia but with elevated high-sensitivity C-reactive protein levels, Crestor significantly reduced the incidence of major cardiovascular incidents as well as lowering CRP levels . Crestor has been shown to increase circulating EPC levels in vivo , in part through reduction of detrimental effects of asymmetric dimethylarginine on EPC .
Besides attempting to reduce inflammation, administration of EPC is another therapeutic possibility. The area of cardiac regeneration has been subject to most stem cell investigation besides hematopoietic reconstitution. Specifically, several double blind studies have been performed demonstrating overall increased cardiac function and reduction in pathological remodeling subsequent to administration of autologous bone marrow mononuclear cells [127–129]. Original thoughts regarding the use of bone marrow stem cells in infarcts revolved around studies showing "transdifferentiation" of various bone marrow derived cells into cells with myocardial features [130, 131]. While this concept is attractive, it has become very controversial in light of several studies demonstrating extremely minute levels of donor-derived cardiomyocytes, despite clinical improvement [132, 133]. An idea that has attracted interest is that bone marrow cells contain high numbers of EPC , so the therapeutic effect post infarct may not necessarily need to be solely based on regeneration via transdifferentiation, but via production of new blood vessels in the injured myocardium mediated by administered EPC in the bone marrow . This view is supported by studies demonstrating that administration of EPC in other conditions of injury or fibrotic healing results in reduced tissue damage and organ functionality.
Instead of administering EPC another therapeutic possibility is to "reposition" them or simply to mobilize them from bone marrow sources. As previously discussed, myocardial and cerebral infarcts seem to cause a "natural mobilization", which may be part of the endogenous response to injury. These observations led investigators to assess whether agents that mobilize EPC may be used therapeutically. Granulocyte colony stimulating factor (G-CSF) has been used clinically for mobilization of hematopoietic stem cells (HSC) for more than a decade during donor stem cell harvesting. Mechanistically G-CSF is believed to induce a MMP-dependent alteration of the SDF-1 gradient in the bone marrow [136, 137], as well as function through a complement-dependent remodeling of the bone marrow extracellular matrix [138, 139]. It was found that in addition to mobilizing HSC, G-CSF stimulates mobilization of EPC as well, through mechanisms that are believed to be related [35, 140]. Several studies have been performed in which G-CSF was administered subsequent to infarct. Although it is impossible to state whether the mobilization of HSC or EPC accounted for the beneficial effects, we will overview some of these studies.
The Front-Integrated Revascularization and Stem Cell Liberation in Evolving Acute Myocardial Infarction by Granulocyte Colony-Stimulating Factor (FIRSTLINE-AMI) trial evaluated 30 patients with ST-elevation myocardial infarction treated with control or G-CSF after successful revascularization . Fifteen patients received 6 days of G-CSF at 10 μg/kg body weight, whereas the other 15 received standard care only. Four months after the infarct, the group that received G-CSF possessed a thicker myocardial wall at the area of infarct, as compared to controls. This was sustained over a year. Statistically significant improvements in ejection fraction, as well as inhibition of pathological remodeling was observed in comparison to controls. A larger subsequent study with 114 patients, 56 treated and 58 control demonstrated "no influence on infarct size, left ventricular function, or coronary restenosis" . There may be a variety of reasons to explain the discrepancy between the trials. One most obvious one is that the mobilization was conducted immediately after the heart attack, whereas it may be more beneficial to time the mobilization with the timing of the chemotactic gradient released by the injured myocardium. This has been used to explain discrepancies between similar regenerative medicine trials . Supporting this possibility is a study in which altered dosing was used for the successful improvement in angina . Furthermore, a recent study last year demonstrated that in 41 patients with large anterior wall AMI an improvement in LVEF and diminished pathological remodeling was observed . Thus while more studies are needed for definitive conclusions, it appears that there is an indication that post-infarct mobilization may have a therapeutic role. In the future, other clinically-applicable mobilizers may be evaluated. For example, growth hormone, which is used in "antiaging medicine" has been demonstrated to improve endothelial responsiveness in healthy volunteers , and patients with congestive heart failure , this appears to be mediated through mobilization of endothelial progenitor cells [148, 149].
Conclusions: Nutraceutical Based Mobilization of EPC
One area of recent interest in the biomedical field has been functional foods and nutraceuticals. While it is known that alteration of diet may modulate FMD responses, to our knowledge, little work as been reported on dietary-supplements altering levels of circulating EPC. The nutritional supplement Stem-Kine (Aidan Products, Chandler, AZ) contains: ellagic acid a polyphenol antioxidant found in numerous vegetables and fruits; vitamin D3 which has been shown to mildly increase circulating progenitor cells; beta 1,3 glucan (previous studies have reported administration of various beta glucans to elicit stem cell mobilization ), and a ferment of the bacterium, Lactobacillus fermentum. Lactobacillus fermentum is generally regarded as safe, and has been in the food supply for hundreds of years as a starter culture for the production of sour dough bread and provides for its characteristic sour flavor. Extract of green tea, extract of goji berries, and extract of the root of astragalus were added prior to the fermentation process. Green tea extracts and some components of goji berries are known to mildly stimulate progenitor cell release, and astragalosides and other molecules found in the root of astragalus are known antioxidants that can prevent cellular damage secondary to oxidation. Fermentation is known to increase the bioavailability of minerals, proteins, peptides, antioxidants, flavanols and other organic molecules. Imm-Kine, another Lactobacillus fermentum fermented product that includes beta 1,3, glucan has been safely distributed for 9 years without reported side effects.
We report here data from 6 healthy volunteers supplemented with StemKine (under an approved IRB protocol) for a period of 14 days (two capsules, am, two capsules pm, by mouth--700 mg per capsule). To our knowledge this is the first report of a combination of naturally occurring molecules from food products altering the levels of circulating EPCs in humans.
As seen in Figure 1, an increase in cells expressing VEGFR2 and CD34 was observed, which was maintained for at least 14 days. These data suggest the feasibility of modulating circulating EPC levels using food supplements. Future studies integrating natural products together with regenerative medicine concepts may lead to formulation of novel treatment protocols applicable to age-associated degeneration.
Herrmann J, Lerman A: The Endothelium - the Cardiovascular Health Barometer. Herz. 2008, 33: 343-353. 10.1007/s00059-008-3088-2.
Hamel E: Perivascular nerves and the regulation of cerebrovascular tone. J Appl Physiol. 2006, 100: 1059-1064. 10.1152/japplphysiol.00954.2005.
Saenz de Tejada I, Angulo J, Cellek S, Gonzalez-Cadavid N, Heaton J, Pickard R, Simonsen U: Pathophysiology of erectile dysfunction. J Sex Med. 2005, 2: 26-39. 10.1111/j.1743-6109.2005.20103.x.
Provis JM, Penfold PL, Cornish EE, Sandercoe TM, Madigan MC: Anatomy and development of the macula: specialisation and the vulnerability to macular degeneration. Clin Exp Optom. 2005, 88: 269-281.
Izikki M, Fadel E, Humbert M, Tu L, Zadigue P, Dartevelle P, Simonneau G, Adnot S, Maitre B, Raffestin B, Eddahibi S: Role for dysregulated endothelium- derived FGF2 signaling in progression of pulmonary hypertension. Rev Mal Respir. 2008, 25: 1192-
Pautler EL: The possible role and treatment of deficient microcirculation regulation in age-associated memory impairment. Med Hypotheses. 1994, 42: 363-366. 10.1016/0306-9877(94)90153-8.
McCarron RM, Chen Y, Tomori T, Strasser A, Mechoulam R, Shohami E, Spatz M: Endothelial-mediated regulation of cerebral microcirculation. J Physiol Pharmacol. 2006, 57 (Suppl 11): 133-144.
Nowak JZ: Age-related macular degeneration (AMD): pathogenesis and therapy. Pharmacol Rep. 2006, 58: 353-363.
Chai SJ, Barrett-Connor E, Gamst A: Small-vessel lower extremity arterial disease and erectile dysfunction: The Rancho Bernardo study. Atherosclerosis. 2009, 203: 620-625. 10.1016/j.atherosclerosis.2008.07.034.
Tuder RM, Yun JH: Vascular endothelial growth factor of the lung: friend or foe. Curr Opin Pharmacol. 2008, 8: 255-260. 10.1016/j.coph.2008.03.003.
Kelm M: Flow-mediated dilatation in human circulation: diagnostic and therapeutic aspects. Am J Physiol Heart Circ Physiol. 2002, 282: H1-5.
Celermajer DS, Sorensen KE, Gooch VM, Spiegelhalter DJ, Miller OI, Sullivan ID, Lloyd JK, Deanfield JE: Non-invasive detection of endothelial dysfunction in children and adults at risk of atherosclerosis. Lancet. 1992, 340: 1111-1115. 10.1016/0140-6736(92)93147-F.
Palmer RM, Ferrige AG, Moncada S: Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature. 1987, 327: 524-526. 10.1038/327524a0.
Ahlers BA, Parnell MM, Chin-Dusting JP, Kaye DM: An age-related decline in endothelial function is not associated with alterations in L-arginine transport in humans. J Hypertens. 2004, 22: 321-327. 10.1097/00004872-200402000-00016.
Taddei S, Virdis A, Mattei P, Ghiadoni L, Gennari A, Fasolo CB, Sudano I, Salvetti A: Aging and endothelial function in normotensive subjects and patients with essential hypertension. Circulation. 1995, 91: 1981-1987.
Andrawis N, Jones DS, Abernethy DR: Aging is associated with endothelial dysfunction in the human forearm vasculature. J Am Geriatr Soc. 2000, 48: 193-198.
Ghiadoni L, Cupisti A, Huang Y, Mattei P, Cardinal H, Favilla S, Rindi P, Barsotti G, Taddei S, Salvetti A: Endothelial dysfunction and oxidative stress in chronic renal failure. J Nephrol. 2004, 17: 512-519.
Bilsborough W, Keen H, Taylor A, O'Driscoll GJ, Arnolda L, Green DJ: Anti-tumour necrosis factor-alpha therapy over conventional therapy improves endothelial function in adults with rheumatoid arthritis. Rheumatol Int. 2006, 26: 1125-1131. 10.1007/s00296-006-0147-y.
Roifman I, Sun YC, Fedwick JP, Panaccione R, Buret AG, Liu H, Rostom A, Anderson TJ, Beck PL: Evidence of endothelial dysfunction in patients with inflammatory bowel disease. Clin Gastroenterol Hepatol. 2009, 7: 175-182. 10.1016/j.cgh.2008.10.021.
Hurks R, Eisinger MJ, Goovaerts I, van Gaal L, Vrints C, Weyler J, Hendriks J, van Schil P, Lauwers P: Early endothelial dysfunction in young type 1 diabetics. Eur J Vasc Endovasc Surg. 2009, 37: 611-615. 10.1016/j.ejvs.2009.01.015.
Crisby M, Kublickiene K, Henareh L, Agewall S: Circulating levels of autoantibodies to oxidized low-density lipoprotein and C-reactive protein levels correlate with endothelial function in resistance arteries in men with coronary heart disease. Heart Vessels. 2009, 24: 90-95. 10.1007/s00380-008-1089-y.
Dede DS, Yavuz B, Yavuz BB, Cankurtaran M, Halil M, Ulger Z, Cankurtaran ES, Aytemir K, Kabakci G, Ariogul S: Assessment of endothelial function in Alzheimer's disease: is Alzheimer's disease a vascular disease?. J Am Geriatr Soc. 2007, 55: 1613-1617. 10.1111/j.1532-5415.2007.01378.x.
Chong AY, Blann AD, Patel J, Freestone B, Hughes E, Lip GY: Endothelial dysfunction and damage in congestive heart failure: relation of flow-mediated dilation to circulating endothelial cells, plasma indexes of endothelial damage, and brain natriuretic peptide. Circulation. 2004, 110: 1794-1798. 10.1161/01.CIR.0000143073.60937.50.
Poredos P: Endothelial dysfunction in the pathogenesis of atherosclerosis. Int Angiol. 2002, 21: 109-116.
Listi F, Caruso C, Balistreri CR, Grimaldi MP, Caruso M, Caimi G, Hoffmann E, Lio D, Candore G: PECAM-1/CD31 in infarction and longevity. Ann N Y Acad Sci. 2007, 1100: 132-139. 10.1196/annals.1395.011.
Ballard VL, Edelberg JM: Targets for regulating angiogenesis in the ageing endothelium. Expert Opin Ther Targets. 2007, 11: 1385-1399. 10.1517/1472818.104.22.1685.
Lu C, Hansen E, Sapozhnikova A, Hu D, Miclau T, Marcucio RS: Effect of age on vascularization during fracture repair. J Orthop Res. 2008, 26: 1384-1389. 10.1002/jor.20667.
Rivard A, Berthou-Soulie L, Principe N, Kearney M, Curry C, Branellec D, Semenza GL, Isner JM: Age-dependent defect in vascular endothelial growth factor expression is associated with reduced hypoxia-inducible factor 1 activity. J Biol Chem. 2000, 275: 29643-29647. 10.1074/jbc.M001029200.
Basak GW, Yasukawa S, Alfaro A, Halligan S, Srivastava AS, Min WP, Minev B, Carrier E: Human embryonic stem cells hemangioblast express HLA-antigens. J Transl Med. 2009, 7: 27-10.1186/1479-5876-7-27.
Stump MM, Jordan GL, Debakey ME, Halpert B: Endothelium Grown from Circulating Blood on Isolated Intravascular Dacron Hub. Am J Pathol. 1963, 43: 361-367.
Asahara T, Murohara T, Sullivan A, Silver M, Zee van der R, Li T, Witzenbichler B, Schatteman G, Isner JM: Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997, 275: 964-967. 10.1126/science.275.5302.964.
Takahashi T, Kalka C, Masuda H, Chen D, Silver M, Kearney M, Magner M, Isner JM, Asahara T: Ischemia- and cytokine-induced mobilization of bone marrow-derived endothelial progenitor cells for neovascularization. Nat Med. 1999, 5: 434-438. 10.1038/8462.
Asahara T, Masuda H, Takahashi T, Kalka C, Pastore C, Silver M, Kearne M, Magner M, Isner JM: Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization. Circ Res. 1999, 85: 221-228.
Peichev M, Naiyer AJ, Pereira D, Zhu Z, Lane WJ, Williams M, Oz MC, Hicklin DJ, Witte L, Moore MA, Rafii S: Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors. Blood. 2000, 95: 952-958.
Korbling M, Reuben JM, Gao H, Lee BN, Harris DM, Cogdell D, Giralt SA, Khouri IF, Saliba RM, Champlin RE: Recombinant human granulocyte-colony-stimulating factor-mobilized and apheresis-collected endothelial progenitor cells: a novel blood cell component for therapeutic vasculogenesis. Transfusion. 2006, 46: 1795-1802. 10.1111/j.1537-2995.2006.00985.x.
Timmermans F, Van Hauwermeiren F, De Smedt M, Raedt R, Plasschaert F, De Buyzere ML, Gillebert TC, Plum J, Vandekerckhove B: Endothelial outgrowth cells are not derived from CD133+ cells or CD45+ hematopoietic precursors. Arterioscler Thromb Vasc Biol. 2007, 27: 1572-1579. 10.1161/ATVBAHA.107.144972.
Rehman J, Li J, Orschell CM, March KL: Peripheral blood "endothelial progenitor cells" are derived from monocyte/macrophages and secrete angiogenic growth factors. Circulation. 2003, 107: 1164-1169. 10.1161/01.CIR.0000058702.69484.A0.
Rohde E, Malischnik C, Thaler D, Maierhofer T, Linkesch W, Lanzer G, Guelly C, Strunk D: Blood monocytes mimic endothelial progenitor cells. Stem Cells. 2006, 24: 357-367. 10.1634/stemcells.2005-0072.
Foteinos G, Hu Y, Xiao Q, Metzler B, Xu Q: Rapid endothelial turnover in atherosclerosis-prone areas coincides with stem cell repair in apolipoprotein E-deficient mice. Circulation. 2008, 117: 1856-1863. 10.1161/CIRCULATIONAHA.107.746008.
Werner N, Junk S, Laufs U, Link A, Walenta K, Bohm M, Nickenig G: Intravenous transfusion of endothelial progenitor cells reduces neointima formation after vascular injury. Circ Res. 2003, 93: e17-24. 10.1161/01.RES.0000083812.30141.74.
Wassmann S, Werner N, Czech T, Nickenig G: Improvement of endothelial function by systemic transfusion of vascular progenitor cells. Circ Res. 2006, 99: e74-83. 10.1161/01.RES.0000246095.90247.d4.
Edelberg JM, Tang L, Hattori K, Lyden D, Rafii S: Young adult bone marrow-derived endothelial precursor cells restore aging-impaired cardiac angiogenic function. Circ Res. 2002, 90: E89-93. 10.1161/01.RES.0000020861.20064.7E.
Ceradini DJ, Gurtner GC: Homing to hypoxia: HIF-1 as a mediator of progenitor cell recruitment to injured tissue. Trends Cardiovasc Med. 2005, 15: 57-63. 10.1016/j.tcm.2005.02.002.
Schomig K, Busch G, Steppich B, Sepp D, Kaufmann J, Stein A, Schomig A, Ott I: Interleukin-8 is associated with circulating CD133+ progenitor cells in acute myocardial infarction. Eur Heart J. 2006, 27: 1032-1037. 10.1093/eurheartj/ehi761.
Shintani S, Murohara T, Ikeda H, Ueno T, Honma T, Katoh A, Sasaki K, Shimada T, Oike Y, Imaizumi T: Mobilization of endothelial progenitor cells in patients with acute myocardial infarction. Circulation. 2001, 103: 2776-2779. 10.1161/hc2301.092122.
Andrassy M, Volz HC, Igwe JC, Funke B, Eichberger SN, Kaya Z, Buss S, Autschbach F, Pleger ST, Lukic IK: High-mobility group box-1 in ischemia-reperfusion injury of the heart. Circulation. 2008, 117: 3216-3226. 10.1161/CIRCULATIONAHA.108.769331.
Palumbo R, Galvez BG, Pusterla T, De Marchis F, Cossu G, Marcu KB, Bianchi ME: Cells migrating to sites of tissue damage in response to the danger signal HMGB1 require NF-kappaB activation. J Cell Biol. 2007, 179: 33-40. 10.1083/jcb.200704015.
Gill M, Dias S, Hattori K, Rivera ML, Hicklin D, Witte L, Girardi L, Yurt R, Himel H, Rafii S: Vascular trauma induces rapid but transient mobilization of VEGFR2(+)AC133(+) endothelial precursor cells. Circ Res. 2001, 88: 167-174.
Wojakowski W, Tendera M, Michalowska A, Majka M, Kucia M, Maslankiewicz K, Wyderka R, Ochala A, Ratajczak MZ: Mobilization of CD34/CXCR4+, CD34/CD117+, c-met+ stem cells, and mononuclear cells expressing early cardiac, muscle, and endothelial markers into peripheral blood in patients with acute myocardial infarction. Circulation. 2004, 110: 3213-3220. 10.1161/01.CIR.0000147609.39780.02.
Massa M, Rosti V, Ferrario M, Campanelli R, Ramajoli I, Rosso R, De Ferrari GM, Ferlini M, Goffredo L, Bertoletti A: Increased circulating hematopoietic and endothelial progenitor cells in the early phase of acute myocardial infarction. Blood. 2005, 105: 199-206. 10.1182/blood-2004-05-1831.
Chang LT, Yuen CM, Sun CK, Wu CJ, Sheu JJ, Chua S, Yeh KH, Yang CH, Youssef AA, Yip HK: Role of stromal cell-derived factor-1alpha, level and value of circulating interleukin-10 and endothelial progenitor cells in patients with acute myocardial infarction undergoing primary coronary angioplasty. Circ J. 2009, 73: 1097-1104. 10.1253/circj.CJ-08-0497.
Sobrino T, Hurtado O, Moro MA, Rodriguez-Yanez M, Castellanos M, Brea D, Moldes O, Blanco M, Arenillas JF, Leira R: The increase of circulating endothelial progenitor cells after acute ischemic stroke is associated with good outcome. Stroke. 2007, 38: 2759-2764. 10.1161/STROKEAHA.107.484386.
Dunac A, Frelin C, Popolo-Blondeau M, Chatel M, Mahagne MH, Philip PJ: Neurological and functional recovery in human stroke are associated with peripheral blood CD34+ cell mobilization. J Neurol. 2007, 254: 327-332. 10.1007/s00415-006-0362-1.
Yip HK, Chang LT, Chang WN, Lu CH, Liou CW, Lan MY, Liu JS, Youssef AA, Chang HW: Level and value of circulating endothelial progenitor cells in patients after acute ischemic stroke. Stroke. 2008, 39: 69-74. 10.1161/STROKEAHA.107.489401.
Wu J, Sun Z, Sun HS, Weisel RD, Keating A, Li ZH, Feng ZP, Li RK: Intravenously administered bone marrow cells migrate to damaged brain tissue and improve neural function in ischemic rats. Cell Transplant. 2008, 16: 993-1005. 10.3727/000000007783472435.
Chen ZZ, Jiang XD, Zhang LL, Shang JH, Du MX, Xu G, Xu RX: Beneficial effect of autologous transplantation of bone marrow stromal cells and endothelial progenitor cells on cerebral ischemia in rabbits. Neurosci Lett. 2008, 445: 36-41. 10.1016/j.neulet.2008.08.039.
Deng YB, Ye WB, Hu ZZ, Yan Y, Wang Y, Takon BF, Zhou GQ, Zhou YF: Intravenously administered BMSCs reduce neuronal apoptosis and promote neuronal proliferation through the release of VEGF after stroke in rats. Neurol Res. 2009,
Ware LB, Matthay MA: The acute respiratory distress syndrome. N Engl J Med. 2000, 342: 1334-1349. 10.1056/NEJM200005043421806.
Suratt BT, Cool CD, Serls AE, Chen L, Varella-Garcia M, Shpall EJ, Brown KK, Worthen GS: Human pulmonary chimerism after hematopoietic stem cell transplantation. Am J Respir Crit Care Med. 2003, 168: 318-322. 10.1164/rccm.200301-145OC.
Yamada M, Kubo H, Ishizawa K, Kobayashi S, Shinkawa M, Sasaki H: Increased circulating endothelial progenitor cells in patients with bacterial pneumonia: evidence that bone marrow derived cells contribute to lung repair. Thorax. 2005, 60: 410-413. 10.1136/thx.2004.034058.
Burnham EL, Taylor WR, Quyyumi AA, Rojas M, Brigham KL, Moss M: Increased circulating endothelial progenitor cells are associated with survival in acute lung injury. Am J Respir Crit Care Med. 2005, 172: 854-860. 10.1164/rccm.200410-1325OC.
Lam CF, Liu YC, Hsu JK, Yeh PA, Su TY, Huang CC, Lin MW, Wu PC, Chang PJ, Tsai YC: Autologous transplantation of endothelial progenitor cells attenuates acute lung injury in rabbits. Anesthesiology. 2008, 108: 392-401. 10.1097/ALN.0b013e318164ca64.
Hatada T, Wada H, Nobori T, Okabayashi K, Maruyama K, Abe Y, Uemoto S, Yamada S, Maruyama I: Plasma concentrations and importance of High Mobility Group Box protein in the prognosis of organ failure in patients with disseminated intravascular coagulation. Thromb Haemost. 2005, 94: 975-979.
Becchi C, Pillozzi S, Fabbri LP, Al Malyan M, Cacciapuoti C, Della Bella C, Nucera M, Masselli M, Boncinelli S, Arcangeli A, Amedei A: The increase of endothelial progenitor cells in the peripheral blood: a new parameter for detecting onset and severity of sepsis. Int J Immunopathol Pharmacol. 2008, 21: 697-705.
Liu Y, Song SD, Wang HX: [A clinical study of the serum vascular endothelial growth factor in patients with severe sepsis]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2009, 21: 172-174.
Matsuda N, Hattori Y: Vascular biology in sepsis: pathophysiological and therapeutic significance of vascular dysfunction. J Smooth Muscle Res. 2007, 43: 117-137. 10.1540/jsmr.43.117.
Regnault V, Levy B: Recombinant activated protein C in sepsis: endothelium protection or endothelium therapy?. Crit Care. 2007, 11: 103-10.1186/cc5135.
Rafat N, Hanusch C, Brinkkoetter PT, Schulte J, Brade J, Zijlstra JG, Woude van der FJ, van Ackern K, Yard BA, Beck G: Increased circulating endothelial progenitor cells in septic patients: correlation with survival. Crit Care Med. 2007, 35: 1677-1684. 10.1097/01.CCM.0000269034.86817.59.
Hristov M, Zernecke A, Schober A, Weber C: Adult progenitor cells in vascular remodeling during atherosclerosis. Biol Chem. 2008, 389: 837-844. 10.1515/BC.2008.093.
Zhao Q, Ren H, Zhu D, Han Z: Stem/progenitor cells in liver injury repair and regeneration. Biol Cell. 2009, 101: 557-571.
Sun Y: Myocardial repair/remodelling following infarction: roles of local factors. Cardiovasc Res. 2009, 81: 482-490. 10.1093/cvr/cvn333.
Ogami M, Ikura Y, Ohsawa M, Matsuo T, Kayo S, Yoshimi N, Hai E, Shirai N, Ehara S, Komatsu R: Telomere shortening in human coronary artery diseases. Arterioscler Thromb Vasc Biol. 2004, 24: 546-550. 10.1161/01.ATV.0000117200.46938.e7.
Goldschmidt-Clermont PJ: Loss of bone marrow-derived vascular progenitor cells leads to inflammation and atherosclerosis. Am Heart J. 2003, 146: S5-12. 10.1016/j.ahj.2003.09.002.
Spyridopoulos I, Erben Y, Brummendorf TH, Haendeler J, Dietz K, Seeger F, Kissel CK, Martin H, Hoffmann J, Assmus B: Telomere gap between granulocytes and lymphocytes is a determinant for hematopoetic progenitor cell impairment in patients with previous myocardial infarction. Arterioscler Thromb Vasc Biol. 2008, 28: 968-974. 10.1161/ATVBAHA.107.160846.
Griese DP, Ehsan A, Melo LG, Kong D, Zhang L, Mann MJ, Pratt RE, Mulligan RC, Dzau VJ: Isolation and transplantation of autologous circulating endothelial cells into denuded vessels and prosthetic grafts: implications for cell-based vascular therapy. Circulation. 2003, 108: 2710-2715. 10.1161/01.CIR.0000096490.16596.A6.
Liu F, Liu ZD, Wu N, Cong X, Fei R, Chen HS, Wei L: Transplanted endothelial progenitor cells ameliorate carbon tetrachloride-induced liver cirrhosis in rats. Liver Transpl. 2009, 15: 1092-1100. 10.1002/lt.21845.
Nakamura T, Torimura T, Sakamoto M, Hashimoto O, Taniguchi E, Inoue K, Sakata R, Kumashiro R, Murohara T, Ueno T, Sata M: Significance and therapeutic potential of endothelial progenitor cell transplantation in a cirrhotic liver rat model. Gastroenterology. 2007, 133: 91-107. 10.1053/j.gastro.2007.03.110. e101
Xin Z, Meng W, Ya-Ping H, Wei Z: Different biological properties of circulating and bone marrow endothelial progenitor cells in acute myocardial infarction rats. Thorac Cardiovasc Surg. 2008, 56: 441-448. 10.1055/s-2008-1038879.
Vila V, Martinez-Sales V, Almenar L, Lazaro IS, Villa P, Reganon E: Inflammation, endothelial dysfunction and angiogenesis markers in chronic heart failure patients. Int J Cardiol. 2008, 130: 276-277. 10.1016/j.ijcard.2007.07.010.
von Haehling S, Schefold JC, Lainscak M, Doehner W, Anker SD: Inflammatory biomarkers in heart failure revisited: much more than innocent bystanders. Heart Fail Clin. 2009, 5: 549-560. 10.1016/j.hfc.2009.04.001.
Stenvinkel P: Inflammation in end-stage renal disease--a fire that burns within. Contrib Nephrol. 2005, 149: 185-199. full_text.
Porazko T, Kuzniar J, Kusztal M, Kuzniar TJ, Weyde W, Kuriata-Kordek M, Klinger M: IL-18 is involved in vascular injury in end-stage renal disease patients. Nephrol Dial Transplant. 2009, 24: 589-596. 10.1093/ndt/gfn486.
Nakae H, Zheng YJ, Wada H, Tajimi K, Endo S: Involvement of IL-18 and soluble fas in patients with postoperative hepatic failure. Eur Surg Res. 2003, 35: 61-66. 10.1159/000069395.
Yumoto E, Higashi T, Nouso K, Nakatsukasa H, Fujiwara K, Hanafusa T, Yumoto Y, Tanimoto T, Kurimoto M, Tanaka N, Tsuji T: Serum gamma-interferon-inducing factor (IL-18) and IL-10 levels in patients with acute hepatitis and fulminant hepatic failure. J Gastroenterol Hepatol. 2002, 17: 285-294. 10.1046/j.1440-1746.2002.02690.x.
Petrovic-Rackov L, Pejnovic N: Clinical significance of IL-18, IL-15, IL-12 and TNF-alpha measurement in rheumatoid arthritis. Clin Rheumatol. 2006, 25: 448-452. 10.1007/s10067-005-0106-0.
Leach ST, Messina I, Lemberg DA, Novick D, Rubenstein M, Day AS: Local and systemic interleukin-18 and interleukin-18-binding protein in children with inflammatory bowel disease. Inflamm Bowel Dis. 2008, 14: 68-74. 10.1002/ibd.20272.
Miles EA, Rees D, Banerjee T, Cazzola R, Lewis S, Wood R, Oates R, Tallant A, Cestaro B, Yaqoob P: Age-related increases in circulating inflammatory markers in men are independent of BMI, blood pressure and blood lipid concentrations. Atherosclerosis. 2008, 196: 298-305. 10.1016/j.atherosclerosis.2006.11.002.
Krabbe KS, Pedersen M, Bruunsgaard H: Inflammatory mediators in the elderly. Exp Gerontol. 2004, 39: 687-699. 10.1016/j.exger.2004.01.009.
Svoboda P, Ko SH, Cho B, Yoo SH, Choi SW, Ye SK, Kasai H, Chung MH: Neopterin, a marker of immune response, and 8-hydroxy-2'-deoxyguanosine, a marker of oxidative stress, correlate at high age as determined by automated simultaneous high-performance liquid chromatography analysis of human urine. Anal Biochem. 2008, 383: 236-242. 10.1016/j.ab.2008.09.014.
Blasko I, Knaus G, Weiss E, Kemmler G, Winkler C, Falkensammer G, Griesmacher A, Wurzner R, Marksteiner J, Fuchs D: Cognitive deterioration in Alzheimer's disease is accompanied by increase of plasma neopterin. J Psychiatr Res. 2007, 41: 694-701. 10.1016/j.jpsychires.2006.02.001.
Capri M, Salvioli S, Sevini F, Valensin S, Celani L, Monti D, Pawelec G, De Benedictis G, Gonos ES, Franceschi C: The genetics of human longevity. Ann N Y Acad Sci. 2006, 1067: 252-263. 10.1196/annals.1354.033.
Ventura E, Durant R, Jaussent A, Picot MC, Morena M, Badiou S, Dupuy AM, Jeandel C, Cristol JP: Homocysteine and inflammation as main determinants of oxidative stress in the elderly. Free Radic Biol Med. 2008, 46 (6): 737-44. 10.1016/j.freeradbiomed.2008.11.002.
van Leuven SI, Birjmohun RS, Franssen R, Bisoendial RJ, de Kort H, Levels JH, Basser RL, Meijers JC, Kuivenhoven JA, Kastelein JJ, Stroes ES: ApoAI-phosphatidylcholine infusion neutralizes the atherothrombotic effects of C-reactive protein in humans. J Thromb Haemost. 2008, 7 (2): 347-54. 10.1111/j.1538-7836.2008.03175.x.
Nagaoka T, Kuo L, Ren Y, Yoshida A, Hein TW: C-reactive protein inhibits endothelium-dependent nitric oxide-mediated dilation of retinal arterioles via enhanced superoxide production. Invest Ophthalmol Vis Sci. 2008, 49: 2053-2060. 10.1167/iovs.07-1387.
Butovsky O, Landa G, Kunis G, Ziv Y, Avidan H, Greenberg N, Schwartz A, Smirnov I, Pollack A, Jung S, Schwartz M: Induction and blockage of oligodendrogenesis by differently activated microglia in an animal model of multiple sclerosis. J Clin Invest. 2006, 116: 905-915. 10.1172/JCI26836.
Pickering M, O'Connor JJ: Pro-inflammatory cytokines and their effects in the dentate gyrus. Prog Brain Res. 2007, 163: 339-354. full_text.
Pluchino S, Muzio L, Imitola J, Deleidi M, Alfaro-Cervello C, Salani G, Porcheri C, Brambilla E, Cavasinni F, Bergamaschi A: Persistent inflammation alters the function of the endogenous brain stem cell compartment. Brain. 2008, 131: 2564-2578. 10.1093/brain/awn198.
Fiorito C, Rienzo M, Crimi E, Rossiello R, Balestrieri ML, Casamassimi A, Muto F, Grimaldi V, Giovane A, Farzati B: Antioxidants increase number of progenitor endothelial cells through multiple gene expression pathways. Free Radic Res. 2008, 42: 754-762. 10.1080/10715760802357057.
Ablin JN, Boguslavski V, Aloush V, Elkayam O, Paran D, Caspi D, George J: Effect of anti-TNFalpha treatment on circulating endothelial progenitor cells (EPCs) in rheumatoid arthritis. Life Sci. 2006, 79: 2364-2369. 10.1016/j.lfs.2006.07.035.
Bosello S, Santoliquido A, Zoli A, Di Campli C, Flore R, Tondi P, Ferraccioli G: TNF-alpha blockade induces a reversible but transient effect on endothelial dysfunction in patients with long-standing severe rheumatoid arthritis. Clin Rheumatol. 2008, 27: 833-839. 10.1007/s10067-007-0803-y.
Harley CB, Futcher AB, Greider CW: Telomeres shorten during ageing of human fibroblasts. Nature. 1990, 345: 458-460. 10.1038/345458a0.
Maier JA, Voulalas P, Roeder D, Maciag T: Extension of the life-span of human endothelial cells by an interleukin-1 alpha antisense oligomer. Science. 1990, 249: 1570-1574. 10.1126/science.2218499.
Schnabl B, Purbeck CA, Choi YH, Hagedorn CH, Brenner D: Replicative senescence of activated human hepatic stellate cells is accompanied by a pronounced inflammatory but less fibrogenic phenotype. Hepatology. 2003, 37: 653-664. 10.1053/jhep.2003.50097.
Maier JA, Statuto M, Ragnotti G: Senescence stimulates U937-endothelial cell interactions. Exp Cell Res. 1993, 208: 270-274. 10.1006/excr.1993.1246.
Shelton DN, Chang E, Whittier PS, Choi D, Funk WD: Microarray analysis of replicative senescence. Curr Biol. 1999, 9: 939-945. 10.1016/S0960-9822(99)80420-5.
Parkinson EK, Munro J, Steeghs K, Morrison V, Ireland H, Forsyth N, Fitzsimmons S, Bryce S: Replicative senescence as a barrier to human cancer. Biochem Soc Trans. 2000, 28: 226-233.
Satoh H, Hiyama K, Takeda M, Awaya Y, Watanabe K, Ihara Y, Maeda H, Ishioka S, Yamakido M: Telomere shortening in peripheral blood cells was related with aging but not with white blood cell count. Jpn J Hum Genet. 1996, 41: 413-417. 10.1007/BF01876332.
Metcalfe JA, Parkhill J, Campbell L, Stacey M, Biggs P, Byrd PJ, Taylor AM: Accelerated telomere shortening in ataxia telangiectasia. Nat Genet. 1996, 13: 350-353. 10.1038/ng0796-350.
Chang E, Harley CB: Telomere length and replicative aging in human vascular tissues. Proc Natl Acad Sci USA. 1995, 92: 11190-11194. 10.1073/pnas.92.24.11190.
Caplan BA, Schwartz CJ: Increased endothelial cell turnover in areas of in vivo Evans Blue uptake in the pig aorta. Atherosclerosis. 1973, 17: 401-417. 10.1016/0021-9150(73)90031-2.
Erusalimsky JD, Kurz DJ: Cellular senescence in vivo: its relevance in ageing and cardiovascular disease. Exp Gerontol. 2005, 40: 634-642. 10.1016/j.exger.2005.04.010.
Satoh M, Minami Y, Takahashi Y, Tabuchi T, Itoh T, Nakamura M: Effect of intensive lipid-lowering therapy on telomere erosion in endothelial progenitor cells obtained from patients with coronary artery disease. Clin Sci (Lond). 2009, 116: 827-835. 10.1042/CS20080404.
Farhat N, Thorin-Trescases N, Voghel G, Villeneuve L, Mamarbachi M, Perrault LP, Carrier M, Thorin E: Stress-induced senescence predominates in endothelial cells isolated from atherosclerotic chronic smokers. Can J Physiol Pharmacol. 2008, 86: 761-769. 10.1139/Y08-082.
Kim KS, Kang KW, Seu YB, Baek SH, Kim JR: Interferon-gamma induces cellular senescence through p53-dependent DNA damage signaling in human endothelial cells. Mech Ageing Dev. 2009, 130: 179-188. 10.1016/j.mad.2008.11.004.
Mezzano D, Pais EO, Aranda E, Panes O, Downey P, Ortiz M, Tagle R, Gonzalez F, Quiroga T, Caceres MS: Inflammation, not hyperhomocysteinemia, is related to oxidative stress and hemostatic and endothelial dysfunction in uremia. Kidney Int. 2001, 60: 1844-1850. 10.1046/j.1523-1755.2001.00998.x.
Satoh M, Ishikawa Y, Takahashi Y, Itoh T, Minami Y, Nakamura M: Association between oxidative DNA damage and telomere shortening in circulating endothelial progenitor cells obtained from metabolic syndrome patients with coronary artery disease. Atherosclerosis. 2008, 198: 347-353. 10.1016/j.atherosclerosis.2007.09.040.
Phillips T, Childs AC, Dreon DM, Phinney S, Leeuwenburgh C: A dietary supplement attenuates IL-6 and CRP after eccentric exercise in untrained males. Med Sci Sports Exerc. 2003, 35: 2032-2037. 10.1249/01.MSS.0000099112.32342.10.
Regensteiner JG, Popylisen S, Bauer TA, Lindenfeld J, Gill E, Smith S, Oliver-Pickett CK, Reusch JE, Weil JV: Oral L-arginine and vitamins E and C improve endothelial function in women with type 2 diabetes. Vasc Med. 2003, 8: 169-175. 10.1191/1358863x03vm489oa.
Kalani R, Judge S, Carter C, Pahor M, Leeuwenburgh C: Effects of caloric restriction and exercise on age-related, chronic inflammation assessed by C-reactive protein and interleukin-6. J Gerontol A Biol Sci Med Sci. 2006, 61: 211-217.
Colbert LH, Visser M, Simonsick EM, Tracy RP, Newman AB, Kritchevsky SB, Pahor M, Taaffe DR, Brach J, Rubin S, Harris TB: Physical activity, exercise, and inflammatory markers in older adults: findings from the Health, Aging and Body Composition Study. J Am Geriatr Soc. 2004, 52: 1098-1104. 10.1111/j.1532-5415.2004.52307.x.
Thijssen DH, de Groot PC, Smits P, Hopman MT: Vascular adaptations to 8-week cycling training in older men. Acta Physiol (Oxf). 2007, 190: 221-228. 10.1111/j.1748-1716.2007.01685.x.
Abidov M, Ramazanov A, Jimenez Del Rio M, Chkhikvishvili I: Effect of Blueberin on fasting glucose, C-reactive protein and plasma aminotransferases, in female volunteers with diabetes type 2: double-blind, placebo controlled clinical study. Georgian Med News. 2006, 66-72.
Alexopoulos N, Vlachopoulos C, Aznaouridis K, Baou K, Vasiliadou C, Pietri P, Xaplanteris P, Stefanadi E, Stefanadis C: The acute effect of green tea consumption on endothelial function in healthy individuals. Eur J Cardiovasc Prev Rehabil. 2008, 15: 300-305. 10.1097/HJR.0b013e3282f4832f.
Ridker PM, Danielson E, Fonseca FA, Genest J, Gotto AM, Kastelein JJ, Koenig W, Libby P, Lorenzatti AJ, MacFadyen JG: Rosuvastatin to prevent vascular events in men and women with elevated C-reactive protein. N Engl J Med. 2008, 359: 2195-2207. 10.1056/NEJMoa0807646.
Spiel AO, Mayr FB, Leitner JM, Firbas C, Sieghart W, Jilma B: Simvastatin and rosuvastatin mobilize Endothelial Progenitor Cells but do not prevent their acute decrease during systemic inflammation. Thromb Res. 2008, 123: 108-113. 10.1016/j.thromres.2008.03.007.
Thum T, Tsikas D, Stein S, Schultheiss M, Eigenthaler M, Anker SD, Poole-Wilson PA, Ertl G, Bauersachs J: Suppression of endothelial progenitor cells in human coronary artery disease by the endogenous nitric oxide synthase inhibitor asymmetric dimethylarginine. J Am Coll Cardiol. 2005, 46: 1693-1701. 10.1016/j.jacc.2005.04.066.
Singh S, Arora R, Handa K, Khraisat A, Nagajothi N, Molnar J, Khosla S: Stem cells improve left ventricular function in acute myocardial infarction. Clin Cardiol. 2009, 32: 176-180. 10.1002/clc.20470.
Martin-Rendon E, Brunskill SJ, Hyde CJ, Stanworth SJ, Mathur A, Watt SM: Autologous bone marrow stem cells to treat acute myocardial infarction: a systematic review. Eur Heart J. 2008, 29: 1807-1818. 10.1093/eurheartj/ehn220.
Abdel-Latif A, Bolli R, Tleyjeh IM, Montori VM, Perin EC, Hornung CA, Zuba-Surma EK, Al-Mallah M, Dawn B: Adult bone marrow-derived cells for cardiac repair: a systematic review and meta-analysis. Arch Intern Med. 2007, 167: 989-997. 10.1001/archinte.167.10.989.
Rota M, Kajstura J, Hosoda T, Bearzi C, Vitale S, Esposito G, Iaffaldano G, Padin-Iruegas ME, Gonzalez A, Rizzi R: Bone marrow cells adopt the cardiomyogenic fate in vivo. Proc Natl Acad Sci USA. 2007, 104: 17783-17788. 10.1073/pnas.0706406104.
Kajstura J, Rota M, Whang B, Cascapera S, Hosoda T, Bearzi C, Nurzynska D, Kasahara H, Zias E, Bonafe M: Bone marrow cells differentiate in cardiac cell lineages after infarction independently of cell fusion. Circ Res. 2005, 96: 127-137. 10.1161/01.RES.0000151843.79801.60.
Psaltis PJ, Zannettino AC, Worthley SG, Gronthos S: Concise review: mesenchymal stromal cells: potential for cardiovascular repair. Stem Cells. 2008, 26: 2201-2210. 10.1634/stemcells.2008-0428.
Norol F, Bonnet N, Peinnequin A, Chretien F, Legrand R, Isnard R, Herodin F, Baillou C, Delache B, Negre D: GFP-transduced CD34+ and Lin- CD34- hematopoietic stem cells did not adopt a cardiac phenotype in a nonhuman primate model of myocardial infarct. Exp Hematol. 2007, 35: 653-661. 10.1016/j.exphem.2006.12.003.
Devanesan AJ, Laughlan KA, Girn HR, Homer-Vanniasinkam S: Endothelial progenitor cells as a therapeutic option in peripheral arterial disease. Eur J Vasc Endovasc Surg. 2009, 38: 475-481. 10.1016/j.ejvs.2009.05.019.
Jujo K, Ii M, Losordo DW: Endothelial progenitor cells in neovascularization of infarcted myocardium. J Mol Cell Cardiol. 2008, 45: 530-544. 10.1016/j.yjmcc.2008.08.003.
Jin F, Zhai Q, Qiu L, Meng H, Zou D, Wang Y, Li Q, Yu Z, Han J, Zhou B: Degradation of BM SDF-1 by MMP-9: the role in G-CSF-induced hematopoietic stem/progenitor cell mobilization. Bone Marrow Transplant. 2008, 42: 581-588. 10.1038/bmt.2008.222.
Carion A, Benboubker L, Herault O, Roingeard F, Degenne M, Senecal D, Desbois I, Colombat P, Charbord P, Binet C, Domenech J: Stromal-derived factor 1 and matrix metalloproteinase 9 levels in bone marrow and peripheral blood of patients mobilized by granulocyte colony-stimulating factor and chemotherapy. Relationship with mobilizing capacity of haematopoietic progenitor cells. Br J Haematol. 2003, 122: 918-926. 10.1046/j.1365-2141.2003.04545.x.
Ratajczak MZ, Wysoczynski M, Reca R, Wan W, Zuba-Surma EK, Kucia M, Ratajczak J: A pivotal role of activation of complement cascade (CC) in mobilization of hematopoietic stem/progenitor cells (HSPC). Adv Exp Med Biol. 2008, 632: 47-60.
Lee HM, Wu W, Wysoczynski M, Liu R, Zuba-Surma EK, Kucia M, Ratajczak J, Ratajczak MZ: Impaired mobilization of hematopoietic stem/progenitor cells in C5-deficient mice supports the pivotal involvement of innate immunity in this process and reveals novel promobilization effects of granulocytes. Leukemia. 2009, 23 (11): 2052-62. 10.1038/leu.2009.158.
Pitchford SC, Furze RC, Jones CP, Wengner AM, Rankin SM: Differential mobilization of subsets of progenitor cells from the bone marrow. Cell Stem Cell. 2009, 4: 62-72. 10.1016/j.stem.2008.10.017.
Ince H, Petzsch M, Kleine HD, Eckard H, Rehders T, Burska D, Kische S, Freund M, Nienaber CA: Prevention of left ventricular remodeling with granulocyte colony-stimulating factor after acute myocardial infarction: final 1-year results of the Front-Integrated Revascularization and Stem Cell Liberation in Evolving Acute Myocardial Infarction by Granulocyte Colony-Stimulating Factor (FIRSTLINE-AMI) Trial. Circulation. 2005, 112: I73-80. 10.1161/CIRCULATIONAHA.105.541433.
Zohlnhofer D, Ott I, Mehilli J, Schomig K, Michalk F, Ibrahim T, Meisetschlager G, von Wedel J, Bollwein H, Seyfarth M: Stem cell mobilization by granulocyte colony-stimulating factor in patients with acute myocardial infarction: a randomized controlled trial. JAMA. 2006, 295: 1003-1010. 10.1001/jama.295.9.1003.
Hill JM, Bartunek J: The end of granulocyte colony-stimulating factor in acute myocardial infarction? Reaping the benefits beyond cytokine mobilization. Circulation. 2006, 113: 1926-1928. 10.1161/CIRCULATIONAHA.106.623777.
Suzuki K, Nagashima K, Arai M, Uno Y, Misao Y, Takemura G, Nishigaki K, Minatoguchi S, Watanabe S, Tei C, Fujiwara H: Effect of granulocyte colony-stimulating factor treatment at a low dose but for a long duration in patients with coronary heart disease. Circ J. 2006, 70: 430-437. 10.1253/circj.70.430.
Leone AM, Galiuto L, Garramone B, Rutella S, Giannico MB, Brugaletta S, Perfetti M, Liuzzo G, Porto I, Burzotta F: Usefulness of granulocyte colony-stimulating factor in patients with a large anterior wall acute myocardial infarction to prevent left ventricular remodeling (the rigenera study). Am J Cardiol. 2007, 100: 397-403. 10.1016/j.amjcard.2007.03.036.
Napoli R, Guardasole V, Angelini V, D'Amico F, Zarra E, Matarazzo M, Sacca L: Acute effects of growth hormone on vascular function in human subjects. J Clin Endocrinol Metab. 2003, 88: 2817-2820. 10.1210/jc.2003-030144.
Napoli R, Guardasole V, Matarazzo M, Palmieri EA, Oliviero U, Fazio S, Sacca L: Growth hormone corrects vascular dysfunction in patients with chronic heart failure. J Am Coll Cardiol. 2002, 39: 90-95. 10.1016/S0735-1097(01)01707-7.
Devin JK, Vaughan DE, Blevins LS, Chen Q, Covington J, Verity DK, Young PP: Low-dose growth hormone administration mobilizes endothelial progenitor cells in healthy adults. Growth Horm IGF Res. 2008, 18: 253-263. 10.1016/j.ghir.2007.11.001.
Thum T, Hoeber S, Froese S, Klink I, Stichtenoth DO, Galuppo P, Jakob M, Tsikas D, Anker SD, Poole-Wilson PA: Age-dependent impairment of endothelial progenitor cells is corrected by growth-hormone-mediated increase of insulin-like growth-factor-1. Circ Res. 2007, 100: 434-443. 10.1161/01.RES.0000257912.78915.af.
Cramer DE, Wagner S, Li B, Liu J, Hansen R, Reca R, Wu W, Surma EZ, Laber DA, Ratajczak MZ, Yan J: Mobilization of hematopoietic progenitor cells by yeast-derived beta-glucan requires activation of matrix metalloproteinase-9. Stem Cells. 2008, 26: 1231-1240. 10.1634/stemcells.2007-0712.
This study was supported in part by Allan P Markin, The Aidan Foundation, and the Center For The Improvement Of Human Functioning International. The authors thank Matthew Gandjian, Victoria Dardov and Famela Ramos for literature searches and critical review of the manuscript.
NHR is a shareholder of Aidan Products. All other authors have no competing interests.
NHR and NAM designed experiments, interpreted data and conceptualized manuscript. RH, JK, KWA, CAS, BM, ANP, MPM, LS, DTA, and TEI provided detailed ideas and discussions, and/or writing of the manuscript. NAM and JAJ performed the experiments. All authors read and approved the final manuscript.
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Mikirova, N.A., Jackson, J.A., Hunninghake, R. et al. Circulating endothelial progenitor cells: a new approach to anti-aging medicine?. J Transl Med 7, 106 (2009). https://doi.org/10.1186/1479-5876-7-106
- Vascular Endothelial Growth Factor
- Granulocyte Colony Stimulate Factor
- Flow Mediate Dilation
- Bone Marrow Mononuclear Cell