Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor antagonist

Table 2 Percent Inhibition of monocyte internalization of AL488-MCP-1 with a CCR2 inhibitor

CCR2 inhibitior (μg/ml)	Vol A	Vol B	Vol C	Vol D	Average	Stdev	%CV
555.560	96.50	95.48	95.49	95.16	95.66	0.58	0.61
185.187	95.51	95.44	94.25	94.53	94.93	0.64	0.67
61.729	92.82	92.76	92.64	93.63	92.96	0.45	0.49
20.576	90.89	91.16	90.10	90.00	90.54	0.58	0.64
6.859	84.82	86.28	85.47	86.23	85.70	0.69	0.81
2.286	78.30	76.71	81.54	79.55	79.03	2.04	2.58
0.762	71.21	75.22	71.71	69.85	72.00	2.29	3.18
0.254	59.49	67.06	62.80	59.43	62.20	3.61	5.80
0.085	43.23	57.00	49.56	47.31	49.28	5.78	11.73
0.028	31.18	38.57	36.08	35.06	35.22	3.07	8.72
0.009	23.76	24.64	21.35	24.84	23.65	1.60	6.77
0.005	13.81	15.68	11.31	13.44	13.56	1.79	13.22

ISSN: 1479-5876