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Table 2 Results of tumor cell purging in mobilized blood cells before and after serial purging steps

From: Molecular purging of multiple myeloma cells by ex-vivo culture and retroviral transduction of mobilized-blood CD34+ cells

Tumor cell frequency

Log decrease

Exp.

Mobilized blood aphereses

CD34+ selected fresh cells

CD34+ cells after culture

NGFR+ selected cells

MM 3

< 1:49334

< 1:49334

N.D.

< 1:49334

 

0

0

-

0

MM 6 #

1:2449

1:11791

< 1:89606

< 1:89606

 

0

0.68

> 1.56

> 1.56

MM 10

> 1:6211

> 1:28818

N.D.

1:28852

 

0

< 0.67

-

0.67

MM 12 #

1:4239

< 1:89606

< 1:89606

< 1:89606

 

0

> 1.32

> 1.32

> 1.32

MM 13 #

1:10646

< 1:89606

< 1:89606

< 1:89606

 

0

> 0.92

> 0.92

> 0.92

MM 14 #

1:490

1:11162

< 1:109697

< 1:109697

 

0

1.36

> 2.35

> 2.35

MM 15 #

N.D.

1:12789

1:2669

1:26667

 

N.D.

0

0

1

MM 16

N.D.

> 1:12422

1:28852

1:89256

 

N.D.

0

0.37

0.86

MM 18 #

N.D.

1:238

< 1:44823

< 1:44823

 

N.D.

0

> 2.27

> 2.27

MM 19

N.D.

1:89286

< 1:89606

< 1:89606

 

N.D.

0

> 0

> 0

POOLED DATA

1:5291

1:12270

1:84602

1:208333

 

0

0.36

1.2

1.59

  1. Mobilized blood cells were analyzed after different purging steps for tumor load, where the specific MM marker was available. DNA from 1 × 104, or 2 × 104 cells was amplified to detect MM contaminants. For 6 patients (#) serial dilutions of DNA were performed, with 0.5 logs step dilutions. The quantification of malignant cells in each cell fraction was calculated according to the single-hit Poisson model, and expressed as tumor cell frequency (1:x). The logarithmic decrease (lower part of cells) was calculated with respect to the foregoing unpurged sample indicated with "0". When the frequency of tumor contamination was below the detection threshold of the system, PCR were scored as negative (clear-shaded cells). The pooled data represent frequency determinations, according to the statistical method by Taswell.
  2. N.D. = not done
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Journal of Translational Medicine

ISSN: 1479-5876

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