- Open Access
HLA-A24 and survivin: possibilities in therapeutic vaccination against cancer
© Andersen et al; licensee BioMed Central Ltd. 2006
- Received: 13 June 2006
- Accepted: 04 September 2006
- Published: 04 September 2006
Recently, it was described that an HLA-A24 restricted peptide derived from the survivin splice variant survivin-2B can be recognized by CD8(+) cytotoxic T-cells. The identification of an HLA-A24 epitope is critical for survivin-based immunotherapy as HLA-24 is the most frequent HLA allele in Asia. Consequently, this survivin-2B epitope is already a target in a clinical study in patients with advanced or recurrent colorectal cancer expressing survivin. However, the splice variant survivin-2B has been described to be pro-apoptotic, and is only expressed at low levels in most malignant tissues. Furthermore, survivin-2B expression are significantly decreased in later tumor stages and inversely correlated with tumor differentiation and invasion. Consequently, survivin is a more general vaccination candidate than the splice variant survivin-2B. Here, we on the basis of spontaneous immune responses in HLA-A24+ cancer patients describes that a HLA-A24-restricted survivin epitopes does indeed exist. Consequently, this epitope is an attractive target for the ongoing survivin-based peptide immunotherapy against cancer.
- Peripheral Blood Lymphocyte
- Recurrent Colorectal Cancer
- Peptide Base Immunotherapy
- Spontaneous Immune Response
- Survivin Epitope
The group of Sato recently attempted to identify a HLA-A24-restricted epitope derived from the universal tumor antigen survivin. Although unsuccessful, they reported that an HLA-A24 restricted peptide (AYACNTSTL) derived from the survivin splice variant survivin-2B can be recognized by CD8(+) cytotoxic T-cells [1, 2]. Subsequently, as described in this journal this survivin-2B epitope is the target in a phase I clinical study assessing the efficacy of survivin-2B peptide vaccination in patients with advanced or recurrent colorectal cancer expressing survivin .
As HLA-A24 is a very frequent allele expressed especially in the Asian population a survivin-derived HLA-A24 restricted epitope would be highly interesting in peptide based immunotherapy against cancer. Survivin is an apoptotic inhibitor that is expressed at high levels in a variety of malignancies. At present, four splicing variants are known for survivin (Survivin, Survivin-2B, Survivin-deltaEx3, Survivin-3B, and survivin-2α) [4–6]. Survivin is over-expressed in almost all cancers including lung, colon, breast, pancreas, stomach, liver, ovaries, and prostate cancer, as well as melanoma, and hematopoetic malignancies [7–9]. Data from a large analysis of human transcripts revealed survivin as the fourth most highly expressed protein in human cancer tissue compared to normal tissue . In contrast, the expression and function in human cancer of the splice variant the survivin-2B is more indistinct. Thus, most studies describe survivin 2-B as to be pro-apoptotic, and only to be expressed at low levels in most malignant tissues . Furthermore, survivin-2B expression are significantly decreased in later tumor stages and inversely correlated with tumor differentiation and invasion [4, 11]. Likewise, Islam et al (2000) reported that the expression of survivin-2B is predominant in some neuroblastoma with a good prognosis , indicating a possible unfavourable role of survivin-2B in cancer development . In contrast, two recent papers show that high expression of survivin-2B variant is correlated with poor prognosis in cancer patients [14, 15]. However, as survivin-2B is not expressed in many malignancies, survivin is a more attractive candidate as a general vaccination candidate than the splice variant survivin-2B. In the present manuscript, we identified an HLA-A24 restricted epitope derived from the universal tumor antigen survivin.
Peripheral blood lymphocytes (PBL) from HLA-A24 positive cancer patients were obtained from the University Hospital in Herlev, Denmark. The PBL were isolated using Lymphoprep separation and cryopreserved in FCS with 10% DMSO. Tissue typing was conducted at the Department of Clinical Immunology, The State Hospital, Copenhagen, Denmark. Informed consent was obtained from the patients before any of theses measures.
Interferon-γ and perforin ELISPOT
The interferon-γ and Perforin ELISPOT assays was used to quantify peptide epitope-specific interferon-γ and perforin releasing effector cells, respectively as described previously [16, 17]. Briefly, nitrocellulosebottomed 96-well plates (MultiScreen MAIP N45; Millipore) were coated with 7.5 μg/ml capture anti-human interferon-γ (Pf-80/164, Mabtech, Sweden) or 30 μg/ml coating anti-human perforin (Pf-80/164, Mabtech) in 100 μl sterile PBS per well. The wells were washed and T2-A24 cells (a kind from Yasuto Akiyama, Tokyo) and effector cells were added with or without 10 μM peptide. In some assays isolated, CD8 positive T-cells were used as effector cells using CD8+ MACS microbeads (Miltenyi Biotec GmbH, Gladbach, Germany). After incubation (37°C/5%CO2) medium was discarded and the wells were washed prior to addition of the secondary detection Ab, anti-human biotinylated interferon-γ (Mabtech) at 2 μg/ml in 75 μl PSB/BSA per well or anti-human biotinylated perforin (Pf-344-biotin, Mabtech) at 1 μg/ml in 100 μl PSB, respectively. The plates were incubated at RT for 2 h, washed, and 100 μl of streptavidin-ALP (Mabtech) diluted 1:1000 in PBS, was added to each well and the plates were incubated for one hour at RT and the enzyme substrate NBT/BCIP (Invitrogen Life Technologies) was added to each well. Upon appearance of dark spots, the reaction was terminated by washing with tap water. The spots were counted using the ImmunoSpot Series 2.0 Analyzer (CTL Analyzers).
Thus, the Sur20–28 reacting cells in the patients PBL are indeed cytotoxic, CD8+ effector cells. Consequently, Sur20–28 is a very attractive target to be included in the ongoing survivin-based peptide immunotherapy against cancer.
- Hirohashi Y, Torigoe T, Maeda A, Nabeta Y, Kamiguchi K, Sato T, Yoda J, Ikeda H, Hirata K, Yamanaka N, Sato N: An HLA-A24-restricted Cytotoxic T Lymphocyte Epitope of a Tumor-associated Protein, Survivin. Clin Cancer Res. 2002, 8: 1731-1739.PubMedGoogle Scholar
- Idenoue S, Hirohashi Y, Torigoe T, Sato Y, Tamura Y, Hariu H, Yamamoto M, Kurotaki T, Tsuruma T, Asanuma H, Kanaseki T, Ikeda H, Kashiwagi K, Okazaki M, Sasaki K, Sato T, Ohmura T, Hata F, Yamaguchi K, Hirata K, Sato N: A potent immunogenic general cancer vaccine that targets survivin, an inhibitor of apoptosis proteins. Clin Cancer Res. 2005, 11: 1474-1482. 10.1158/1078-0432.CCR-03-0817.View ArticlePubMedGoogle Scholar
- Tsuruma T, Hata F, Torigoe T, Furuhata T, Idenoue S, Kurotaki T, Yamamoto M, Yagihashi A, Ohmura T, Yamaguchi K, Katsuramaki T, Yasoshima T, Sasaki K, Mizushima Y, Minamida H, Kimura H, Akiyama M, Hirohashi Y, Asanuma H, Tamura Y, Shimozawa K, Sato N, Hirata K: Phase I clinical study of anti-apoptosis protein, survivin-derived peptide vaccine therapy for patients with advanced or recurrent colorectal cancer. J Transl Med. 2004, 2: 19-10.1186/1479-5876-2-19.PubMed CentralView ArticlePubMedGoogle Scholar
- Mahotka C, Wenzel M, Springer E, Gabbert HE, Gerharz CD: Survivin-deltaEx3 and survivin-2B: two novel splice variants of the apoptosis inhibitor survivin with different antiapoptotic properties. Cancer Res. 1999, 59: 6097-6102.PubMedGoogle Scholar
- Badran A, Yoshida A, Ishikawa K, Goi T, Yamaguchi A, Ueda T, Inuzuka M: Identification of a novel splice variant of the human anti-apoptopsis gene survivin. Biochem Biophys Res Commun. 2004, 314: 902-907. 10.1016/j.bbrc.2003.12.178.View ArticlePubMedGoogle Scholar
- Caldas H, Honsey LE, Altura RA: Survivin 2alpha: a novel Survivin splice variant expressed in human malignancies. Mol Cancer. 2005, 4: 11-10.1186/1476-4598-4-11.PubMed CentralView ArticlePubMedGoogle Scholar
- Ambrosini G, Adida C, Sirugo G, Altieri DC: Induction of apoptosis and inhibition of cell proliferation by survivin gene targeting. J Biol Chem. 1998, 273: 11177-11182. 10.1074/jbc.273.18.11177.View ArticlePubMedGoogle Scholar
- Adida C, Haioun C, Gaulard P, Lepage E, Morel P, Briere J, Dombret H, Reyes F, Diebold J, Gisselbrecht C, Salles G, Altieri DC, Molina TJ: Prognostic significance of survivin expression in diffuse large B-cell lymphomas. Blood. 2000, 96: 1921-1925.PubMedGoogle Scholar
- Grossman D, McNiff JM, Li F, Altieri DC: Expression and targeting of the apoptosis inhibitor, survivin, in human melanoma. J Invest Dermatol. 1999, 113: 1076-1081. 10.1046/j.1523-1747.1999.00776.x.View ArticlePubMedGoogle Scholar
- Velculescu VE, Madden SL, Zhang L, Lash AE, Yu J, Rago C, Lal A, Wang CJ, Beaudry GA, Ciriello KM, Cook BP, Dufault MR, Ferguson AT, Gao Y, He TC, Hermeking H, Hiraldo SK, Hwang PM, Lopez MA, Luderer HF, Mathews B, Petroziello JM, Polyak K, Zawel L, Kinzler KW, .: Analysis of human transcriptomes. Nat Genet. 1999, 23: 387-388. 10.1038/70487.View ArticlePubMedGoogle Scholar
- Li F: Role of survivin and its splice variants in tumorigenesis. Br J Cancer. 2005, 92: 212-216.PubMed CentralPubMedGoogle Scholar
- Islam A, Kageyama H, Takada N, Kawamoto T, Takayasu H, Isogai E, Ohira M, Hashizume K, Kobayashi H, Kaneko Y, Nakagawara A: High expression of Survivin, mapped to 17q25, is significantly associated with poor prognostic factors and promotes cell survival in human neuroblastoma. Oncogene. 2000, 19: 617-623. 10.1038/sj.onc.1203358.View ArticlePubMedGoogle Scholar
- Meng H, Lu C, Mabuchi H, Tanigawa N: Prognostic significance and different properties of survivin splicing variants in gastric cancer. Cancer Lett. 2004, 216: 147-155. 10.1016/j.canlet.2003.12.020.View ArticlePubMedGoogle Scholar
- Taubert H, Kappler M, Bache M, Bartel F, Kohler T, Lautenschlager C, Blumke K, Wurl P, Schmidt H, Meye A, Hauptmann S: Elevated expression of survivin-splice variants predicts a poor outcome for soft-tissue sarcomas patients. Oncogene. 2005, 24: 5258-5261. 10.1038/sj.onc.1208702.View ArticlePubMedGoogle Scholar
- Wagner M, Schmelz K, Wuchter C, Ludwig WD, Dorken B, Tamm I: In vivo expression of survivin and its splice variant survivin-2B: impact on clinical outcome in acute myeloid leukemia. Int J Cancer. 2006, 119: 1291-1297. 10.1002/ijc.21995.View ArticlePubMedGoogle Scholar
- Andersen MH, Pedersen LO, Capeller B, Brocker EB, Becker JC, thor Straten P: Spontaneous cytotoxic T-cell responses against survivin-derived MHC class I-restricted T-cell epitopes in situ as well as ex vivo in cancer patients. Cancer Res. 2001, 61: 5964-5968.PubMedGoogle Scholar
- Zuber B, Levitsky V, Jonsson G, Paulie S, Samarina A, Grundstrom S, Metkar S, Norell H, Callender GG, Froelich C, Ahlborg N: Detection of human perforin by ELISpot and ELISA: ex vivo identification of virus-specific cells. J Immunol Methods. 2005, 302: 13-25. 10.1016/j.jim.2005.04.015.View ArticlePubMedGoogle Scholar
- Bachinsky MM, Guillen DE, Patel SR, Singleton J, Chen C, Soltis DA, Tussey LG: Mapping and binding analysis of peptides derived from the tumor-associated antigen survivin for eight HLA alleles. Cancer Immun. 2005, 5:6.: 6-Google Scholar
- Andersen MH, Pedersen LO, Becker JC, thor Straten P: Identification of a Cytotoxic T Lymphocyte Response to the Apoptose Inhibitor Protein Survivin in Cancer Patients. Cancer Res. 2001, 61: 869-872.PubMedGoogle Scholar
- Andersen MH, Reker S, Becker JC, thor Straten P: The melanoma inhibitor of apoptosis protein: a target for spontaneous cytotoxic T cell responses. J Invest Dermatol. 2004, 122: 392-399. 10.1046/j.0022-202X.2004.22242.x.View ArticlePubMedGoogle Scholar
- Andersen MH, Svane IM, Kvistborg P, Nielsen OJ, Balslev E, Reker S, Becker JC, Thor SP: Immunogenicity of Bcl-2 in cancer patients. Blood. 2005, 15: 728-734. 10.1182/blood-2004-07-2548.View ArticleGoogle Scholar
- Scheibenbogen C, Sun Y, Keilholz U, Song M, Stevanovic S, Asemissen AM, Nagorsen D, Thiel E, Rammensee HG, Schadendorf D: Identification of known and novel immunogenic T-cell epitopes from tumor antigens recognized by peripheral blood T cells from patients responding to IL-2-based treatment. Int J Cancer. 2002, 20;98: 409-414. 10.1002/ijc.10205.View ArticleGoogle Scholar
- Herr W, Schneider J, Lohse AW, Meyer zum Buschenfelde KH, Wolfel T: Detection and quantification of blood-derived CD8+ T lymphocytes secreting tumor necrosis factor alpha in response to HLA-A2.1-binding melanoma and viral peptide antigens. J Immunol Methods. 1996, 191: 131-142. 10.1016/0022-1759(96)00007-5.View ArticlePubMedGoogle Scholar
- Nagorsen D, Keilholz U, Rivoltini L, Schmittel A, Letsch A, Asemissen AM, Berger G, Buhr HJ, Thiel E, Scheibenbogen C: Natural T-cell response against MHC class I epitopes of epithelial cell adhesion molecule, her-2/neu, and carcinoembryonic antigen in patients with colorectal cancer. Cancer Res. 2000, 60: 4850-4854.PubMedGoogle Scholar
- Valmori D, Dutoit V, Rubio-Godoy V, Chambaz C, Lienard D, Guillaume P, Romero P, Cerottini JC, Rimoldi D: Frequent cytolytic T-cell responses to peptide MAGE-A10(254-262) in melanoma. Cancer Res. 2001, 61: 509-512.PubMedGoogle Scholar
- Ikeda-Moore Y, Tomiyama H, Miwa K, Oka S, Iwamoto A, Kaneko Y, Takiguchi M: Identification and characterization of multiple HLA-A24-restricted HIV-1 CTL epitopes: strong epitopes are derived from V regions of HIV-1. J Immunol. 1997, 159: 6242-6252.PubMedGoogle Scholar
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