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Table 1

From: A novel method of modifying immune responses by vaccination with lipiodol-siRNA mixtures

 

Surface molecules (MFI)

 

CD80

CD86

CD40

Unactivated DC

   

Media Control

571.7 (± 23)

1194 (± 125)

29.5 (± 1.5)

Lipiodol Control

567.3 (± 21)

1251 (± 173)

28.6 (± 1.3)

Mixed siRNA

572.5 (± 10)

1365 (± 157)

29.7 (± 1.3)

siRNA alone

559.6 (± 33)

1201 (± 193)

30.1 (± 0.9)

siRNA + Lipiodol

577.3 (± 15)

1195 (± 103)

30.4 (± 1.3)

Post-MLR

   

Lipiodol Control

1962.9 (± 136)

2707.1 (± 576)

140.5 (± 35.4)

Mixed siRNA

1933.2 (± 324)

3031.6 (± 489)

170.3 (± 26.9)

siRNA alone

1905.7 (± 351)

2759.9 (± 392)

167.2 (± 46.9)

siRNA + Lipiodol

2050.8 (± 119)

3051.1 (± 335)

153.3 (± 36.7)

Post-MLR + Anti-IL10

   

Lipiodol Control

3529 (± 302)

4862 (± 583)

236.0 (± 47.3)

Mixed siRNA

3442 (± 247)

4777 (± 773)

242.8 (± 58.9)

siRNA alone

3723 (± 453)

4863 (± 669)

237.4 (± 43.5)

siRNA + Lipiodol

3985 (± 339)

4957 (± 501)

254.3 (± 49.3)

  1. Surface staining for FACS analysis was performed using anti-CD11c Ab (gated DC) and anti-CD80, CD86 or CD40 Abs (MFI values). Samples stained with the appropriate isotype-matched control Abs were analyzed in parallel to establish the gating criterion. Results are representative of at least three independent experiments for each culture condition.