Epitope definition by proteomic similarity analysis: identification of the linear determinant of the anti-Dsg3 MAb 5H10

Table 1 Sequence-specific assignments in the ¹⁵N-labelled Dsg3_49–60R EWV KFA KPCR E peptide, and chemical shift changes on MAb 5H10 or 5G11 binding.

The ¹⁵N-amino acid position in the peptide is reported. Theoretical chemical shift values were derived from http://www.bmrb.wisc.edu/ref_info/statful.htm and corrected [29]. Experimental chemical shift values were derived from resonance spectra reported in Fig. 4. The average value of chemical shifts relative to the H and N atoms of α amino residue, and the H and N atoms of η amino residues are reported for Arg-11 (data from: http://www.bmrb.wisc.edu/ref_info/statful.htm). The chemical shifts relative to the H and N atoms of α amino residue in Arg-1 are undetectable because of terminal position of the amino acid in the peptide. n.d., not detected.

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