Figure 2

Drug potency against stem cell-like GBM neurosphere derived from patient surgical isolates. (A) Photomicrographs of primary cells growing as adherent culture and neurospheres and expressing the neural stem cell marker Nestin. (B) Eight FDA approved drugs showing best activity in established cell line screens tested in duplicate with adherent cultures and with neurospheres. In each case D-actinomycin exhibited the lowest IC₅₀.