Gene expression analysis of BMSCs co-cultured with CD34+cells compared with BMSC mono-cultures shows changes in metabolism related genes. (A) Hierarchical cluster analysis of 4904 differentially expressed genes in BMSCs co-cultured in transwells with CD34+ cells from three healthy donors compared with BMSC mono-cultures (control) using Partek Genomic Suite program (ANOVA test with unadjusted p-value < 0.05). The displayed colors represent the fold changes with the shades of red and blue indicating up- and down-regulation respectively. The color key for the sample labels is on the top left. (B) Ingenuity Pathway Analysis (IPA) of the 4904 differentially expressed BMSC genes. Numerical symbols at the right side of each bar indicate the total number of genes in each pathway. The bars indicate the percentage of up- (red bar) and down-regulated (green bar) genes in each pathway, while the orange line indicates minus-log transformed p-value. The top 10 canonical pathways are shown.