Figure 5

Immunohistochemical (IHC) staining and Western blot analysis of glucagon-like peptide-1 receptor (GLP-1R) at 24 h and 72 h after ischemia-reperfusion (IR) procedure (n = 6 at each time for each group). A to D) and F to I) The IHC stain (200 x) identifying the GLP-1R (brown color) in kidney parenchyma among four groups at 24 h (A to D) and 72 h (F to I) after IR procedure, respectively. E) for 24 h after IR procedure: * vs. other groups with different symbols (*, †, ‡, §), p < 0.0001. J) for 72 h after IR procedure: * vs. other groups with different symbols (*, †, ‡, §), p < 0.0001. Scale bars in right lower corner represent 50 μm. K and L) The protein expression of GLP-1R in kidney parenchyma among four group at 24 h (K) and 72 h (L) after acute IR injury, respectively; for 24 h after IR: * vs. other groups with different symbols (*, †, ‡, §), p < 0.001; for 72 h after IR: * vs. other groups with different symbols (*, †, ‡, §), p < 0.0001. Symbols (*, †, ‡, §) indicate significance (at 0.05 level). Scale bars in right lower corner represent 50 μm. SC = sham control; IR = ischemia-reperfusion; Sita = sitagliptin; Ex-4 = extendin-4.