Figure 3

Inhibition of miR-329 promotes cell proliferation in glioma. A ) MTT assays revealed that inhibition of miR-329 increased cell growth of glioma cell lines of T98G and LN18. B ) Representative micrographs (left) and quantification (right) of crystal violet stained cell colonies. C ) Inhibition of miR-329 increased the anchorage-independent growth of glioma cells. Representative micrographs (up) and quantification of colonies that were larger than 0.1 mm (down) were scored. D ) Representative micrographs (left) and quantification of BrdU incorporating-cells after transfection with miR-329 inhibitor or NC. E ) Flow cytometric analysis of the indicated glioma cells transfected with NC or miR-329 inhibitor. Each bar represents the mean of three independent experiments. * P < 0.05.