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LPS-induced gene expression of inflammation-related genes in neutrophils from familial Mediterranean fever patients

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Journal of Translational Medicine201210 (Suppl 3) :P56

  • Published:


  • Innate Immune System
  • Familial Mediterranean Fever
  • Neutrophil Activation
  • Familial Mediterranean Fever Patient
  • Bacterial Lipopolysaccharide


Autoinflammatory disorders, such as Familial Mediterranean fever (FMF), characterized by abnormally increased inflammation, and mediated predominantly by the cells of innate immune system. FMF characterized by the episodes of self-resolving severe inflammation, with fever and serositis. The major cell type found in FMF inflammatory exudates are neutrophils. It is not known whether bacterial lipopolysaccharide (LPS) could influence neutrophil activation in FMF. We examined in vitro effect of LPS (10 ng/ml) exposure on expression of 13 selected genes in FMF and control neutrophils using quantitative real-time RT-PCR.


LPS exposure induced basal expression of TLR4 (4.4-fold increase, p<0.05), IL-1β (9-fold, p<0.01), IL-8 (13-fold, p=0.06), TNFAIP6 (11-fold, p<0.05) genes in FMF neutrophils, and of TLR4 (17.6-fold, p<0.001), IL-1β (25-fold, p<0.001), IL-8 (36-fold, p<0.001), TNFAIP6 (61-fold, p<0.001) in healthy neutrophils.


Our findings indicate that gene expression in LPS-exposed FMF and control neutrophils is characterized by a number of shared alterations. In spite of pre-activated state of FMF neutrophils, LPS exposure further enhanced expression of the investigated genes. The ability of FMF neutrophils to enhance LPS-induced inflammatory reaction was however attenuated compared to healthy neutrophils. In conclusion, our data indicate that FMF neutrophils may display features of LPS tolerance, and this speculation should be explored further.


Grant support: IGA_PU_LF_2012_007 and ANSEF NS_1987

Authors’ Affiliations

Group of Molecular and Cellular Immunology, Institute of Molecular Biology, National Academy of Sciences, Yerevan, Armenia
Laboratory of Immunogenomics and Proteomics, Medical Faculty, Palacky University, Olomouc, Czech Republic


© Manukyan et al; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.