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  • Oral presentation
  • Open Access

No lack of regulatory B cells in patients with Multiple Sclerosis

  • 1, 2, 3,
  • 1, 3,
  • 1,
  • 1, 3,
  • 1, 3,
  • 1, 3,
  • 1, 3,
  • 2,
  • 2,
  • 2,
  • 1, 3,
  • 1, 3,
  • 1, 2, 3 and
  • 1, 3
Contributed equally
Journal of Translational Medicine201210 (Suppl 3) :O4

https://doi.org/10.1186/1479-5876-10-S3-O4

  • Published:

Keywords

  • Cell Proliferation
  • Multiple Sclerosis
  • Healthy Volunteer
  • Experimental Autoimmune Encephalomyelitis
  • Disease Symptom

Background

Recent data support a prominent role for B cells in MS physiopathology. Recently it has emerged that subsets of B cells secreting IL-10 negatively regulate disease symptoms in Experimental Autoimmune Encephalomyelitis (EAE). However, the involvement of such regulatory B cells in MS remains unclear.

Aim

We aimed to study the frequency, phenotype and function of regulatory B cells in MS patients as compared to Healthy Volunteers (HV).

Methods

Sixty-three untreated MS patients and 58 HV were included in this study. IL-10 secretion by B cells and phenotype of IL-10+ B cells were studied after 5h (B10 cells) and 48h of stimulation (B10pro cells) by CD40L and ODN. Coculture assays with prestimulated B cells and responding CD4+CD25- T cells were performed for 3 days.

Results

No significant difference was found either for IL-10 secretion ability of B cells after 5h or 48h of stimulation. The analysis of B10pro cells phenotype revealed mainly a memory phenotype in MS and HV, even if both naïve and immature subsets were also able to secrete IL-10. Prestimulated B cells from MS inhibited CD4+CD25- T cell proliferation in the same manner than HV by a contact dependent mechanism, independently of IL-10 and TGF-β secretion.

Conclusion

Altogether, our data show that regulatory B cells have a conserved frequency, phenotype and function in the blood of patients with MS suggesting that B cells do not contribute to the physiopathology of the disease.

Acknowledgements

This work was funded by ARSEP.

Notes

Authors’ Affiliations

(1)
INSERM, UMR, 1064 Nantes, France
(2)
CHU Nord Laennec, Service de Neurologie, Nantes, France
(3)
CHU de Nantes, ITUN, Nantes, France

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