No lack of regulatory B cells in patients with Multiple Sclerosis
- Laure Michel1, 2, 3,
- Mélanie Chesneau1, 3,
- Philippe Manceau1,
- Alexandra Garcia1, 3,
- Marion Salou1, 3,
- Annie Elong Ngono1, 3,
- Annaïck Pallier1, 3,
- Marylène Jacq-Foucher2,
- Fabienne Lefrère2,
- Sandrine Wiertlewski2,
- Jean-Paul Soulillou1, 3,
- Nicolas Degauque1, 3,
- David-Axel Laplaud†1, 2, 3 and
- Sophie Brouard†1, 3
© Michel et al; licensee BioMed Central Ltd. 2012
Published: 28 November 2012
Recent data support a prominent role for B cells in MS physiopathology. Recently it has emerged that subsets of B cells secreting IL-10 negatively regulate disease symptoms in Experimental Autoimmune Encephalomyelitis (EAE). However, the involvement of such regulatory B cells in MS remains unclear.
We aimed to study the frequency, phenotype and function of regulatory B cells in MS patients as compared to Healthy Volunteers (HV).
Sixty-three untreated MS patients and 58 HV were included in this study. IL-10 secretion by B cells and phenotype of IL-10+ B cells were studied after 5h (B10 cells) and 48h of stimulation (B10pro cells) by CD40L and ODN. Coculture assays with prestimulated B cells and responding CD4+CD25- T cells were performed for 3 days.
No significant difference was found either for IL-10 secretion ability of B cells after 5h or 48h of stimulation. The analysis of B10pro cells phenotype revealed mainly a memory phenotype in MS and HV, even if both naïve and immature subsets were also able to secrete IL-10. Prestimulated B cells from MS inhibited CD4+CD25- T cell proliferation in the same manner than HV by a contact dependent mechanism, independently of IL-10 and TGF-β secretion.
Altogether, our data show that regulatory B cells have a conserved frequency, phenotype and function in the blood of patients with MS suggesting that B cells do not contribute to the physiopathology of the disease.
This work was funded by ARSEP.
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