Phenotypes and clinical significance of circulating CD4+CD25+ regulatory T cells (Tregs) in patients with acute-on-chronic liver failure (ACLF)

Table 2 Frequency of skewed TCRBV families in CD4 ⁺ CD25 ⁺ Treg from patients with ACLF, CHB, and HCs ^a

TCRBV families	ACLF Incidence (%)^b	CHB Incidence (%)^b	HCs Incidence (%)^b
1	3	5	0
2	4	3	1
3	3	5	1
4	5	6	2
5.1	6 (26.1)	3	2
5.2	2	6	1
6	2	5	2
7	5	6	1
8	3	3	2
9	3	2	3
10	5	3	1
11	9 (39.1)	9 (33.3)	2
12	6 (26.1)	6	1
13.1	7 (30.1)	8 (29.6)	2
13.2	3	6	2
14	3	2	1
15	4	8 (29.6)	1
16	3	5	2
17	5	7 (25.9)	1
18	8 (34.8)	9 (33.3)	2
19	2	2	0
20	10 (43.5)	11 (40.7)	2
21	1	5	1
22	3	7 (25.9)	1
23	5	5	0
24	3	5	2
Total no. of skewed Vβ (average ratio for a case)	113 (4.91) ^{c, d}	142 (5.26) ^{c, d}	36 (2.12) ^c
No. of patients examined with normal pattern ^e (ratio,%)	2 (8.0) ^f	3 (10.0) ^f	13 (43.3) ^f
No. of patients examined	25	30	30

^aThe number of TCRBV gene families showing skewed-clone expansion (oligoclonal or monoclonal) is summarized in patients with ALCF, CHB and HCs.
^bThe number of samples showing a skewed-clone expansion in total detected samples (percentages of each TCRBV skewed-clone expansion). Samples with normal GMSP are excluded in the percentage calculation.
^cThe average skewed expansion rate of TCRBV gene families was higher in CD4⁺CD25⁺ Treg from patients with ACLF or CHB than that of HC group (P < 0.01 by χ² test).
^dThere was no significant difference between the two groups (P > 0.05 by χ² test). Samples with normal GMSP are excluded in the average ratio calculation
^eNormal pattern (GMSP) means that there is no monoclonal or oligoclonal pattern in any TCRBV families in a patient.
^fThe incidence of the normal GMSP was significantly lower in CD4⁺CD25⁺ Treg from patients with ACLF or CHB than that of HC group (P < 0.01 by χ² test).

ISSN: 1479-5876