Fig. 6

Gene expression changes in CD22 CAR T-cell products separated based on final pH. Gene expression analysis of the final products cultured either in high or low pH demonstrated in total 78 differentially expressed genes: 15 downregulated and 63 upregulated in low pH condition. Blue point represents downregulated genes. Red point indicates up-regulated genes. Genes with p value < 0.01 & |Foldchange|> 2 were considered as differentially expressed genes. GSEA analysis indicates the activation of the four pathways (lymphocyte activation and differentiation, lymphocyte mediated immunity and T cells activation) in high pH group. A significant positive NES value (with FDR < 0.05) is considered indicative of the activation state of the pathway in high pH group