Fig. 2
From: Deciphering the importance of culture pH on CD22 CAR T-cells characteristics
Extracellular changes in pH induced TNC, fold expansion and metabolites change at the beginning of CD22 CAR T-cells manufacturing process. a 12 samples were preserved in low pH (mean 7.2) whereas 8 samples in high pH (mean 7.4, p < 0.0001). b Higher pH negatively correlated and reduced number of total nucleated cells (236 × 106) on Day 2 of the manufacturing process in comparison to 414 × 106 cultured in lower pH (p < 0.0061). c pH values significantly correlated with T-cell expansion. T cells cultured in lower pH reached significantly higher expansion rate related to the ones sustained in high pH (0.85 vs 0.53, p < 0.0015). d Tension of carbon dioxide significantly correlated with pH and was decreased in high pH (23.2 vs 17.6 mmHg, p < 0.001). e Low pH induced drop in glucose levels (2.3 vs 1.9 g/L, p < 0.003) whereas opposite trend was seen for lactate levels (f) (0.38 vs 0.73, p < 0.0001). Both metabolites showed significant correlation