Fig. 5

AZGP1 protein expression in PC3 cell lines modulates the angiogenesis pathway. A Volcano plot of proteome of PC3 vector control and PC3-AZGP1-OV shows 1282 significantly modulated proteins (grey dots) and 94 were identified as angiogenesis-related genes (red dots). Proteins illustrated in red demonstrate 29 upregulated genes and 25 downregulated genes that are associated with angiogenesis. B Hypergeometric distribution of angiogenesis genes shows that the identified 54 genes are significantly upregulated or downregulated between PC3 control and AZPG1-OV tumors (p = 0.014) C Heatmap of the 54 angiogenesis genes in AZGP1-OV PC3 and controls shows clear segregation of the over-expressing and low expressing cells. D Relative protein expression levels of MMP9, E PDCD6, F EphA2 in AZGP1-OV PC3 and control tumors. G Western blot confirming decreased levels of MMP9 and increased levels of PDCD6 and EphA2 proteins, and validation of AZGP1 overexpression in AZGP1-OV PC3 tumors