Fig. 1

AZGP1 has no impact on cell proliferation in PCa cells. A Transcript expression levels of AZGP1 are higher in LNCaP, C42B, and 22Rv1 cell lines compared to PC3 and DU145 cells, as determined by RT-qPCR. B Western blot analysis of protein levels of AZGP1 are higher in LNCaP, C42B, and 22Rv1 cell lines compared to PC3 and DU145 cells. C Knockout of AZGP1 in LNCaP sg-AZGP1-T1 and sg-AZGP1-T2 cells showed no significant effect on cell proliferation. D Western blot confirming the knockout of AZGP1 in LNCaP sg-AZGP1-T1 and sg-AZGP1-T2 cells. Blot membrane was incubated with secondary antibody overnight. E Overexpression of AZGP1 in LNCaP AZGP1-OV cells did not impact cell proliferation. F Western blot confirming increased AZGP1 protein expression in LNCaP AZGP1-OV cells. The blot membrane was incubated with secondary antibody for 1 h. G Knockout of AZGP1 had no effect on colony formation in LNCaP cells. H Quantification of colony formation in control and AZGP1 knockout sg-AZGP1-T1 and sg-AZGP1-T2 LNCaP cells. I Overexpression of AZGP1 had no effect on colony formation in LNCaP cells. J Quantification of colony formation in LNCaP control and AZGP1-OV cells. K Knockout of AZGP1 had no significant effect on cell proliferation in LuCaP147 cells. L Western blot confirming the knockout of AZGP1 in LuCaP147 sg-AZGP1-T1 and sg-AZGP1-T2 cells. Blot membrane was incubated with secondary antibody overnight. M Overexpression of AZGP1 did not affect cell proliferation in LuCaP147 cells. N Western blot confirming the overexpression of AZGP1 in LuCaP147 AZGP1-OV cells. Blot membrane was incubated with secondary antibody for 1 h. Note: the exposure time for AZPG1 knockout cells (LNCaP control, sg-AZGP1-T1 and sg-AZGP1-T2 cells, LuCaP147 control, sg-AZGP1-T1 and sg-AZGP1-T2 cells,) and AZGP1 overexpression cells (LNCaP control and AZGP1-OV, LuCaP147 control and AZGP1-OV cells) are different