Fig. 4

IL-33 overexpression inhibited adipogenesis in 3T3-L1 cells. 3T3-L1 cells were transfected with LV-IL-33 before adipogenic differentiation, and 72 h later, the cells were incubated for adipogenic differentiation and treated with OA for hyperadipogenic differentiation. (a) qPCR verified the efficiency of IL-33 overexpression in 3T3-L1 cells. (b) On day 10 of adipogenic differentiation, 3T3-L1 cells were stained with Oil Red O; scale bar = 100 μm. (c) Semi-quantitative detection of cells in different groups by Oil Red O staining (detection at 492 nm). (d) Cellular TG levels were measured after 10-day induction. (e) qPCR was conducted to evaluate the expression of the adipogenic factors PPAR-γ, C/EBPα, FABP4, LPL, Adipoq and CD36. The data are represented as the mean ± SEM (n = 3). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, and ^****P < 0.0001. PPAR-γ, Peroxisome proliferator-activated receptor gamma; C/EBPα, CCAAT/enhancer-binding protein α; FABP4, Fatty acid binding protein 4; LPL, lipoprotein lipase; Adipoq, adiponectin; CD36, platelet glycoprotein 4