Fig. 1

Identifying HSPC populations in BM. (A) UMAP of human BM cells from 7 MDS/sAML patients and 3 healthy controls. RS-MLD, ring sideroblasts-multilineage dysplasia; EB-2, excess blasts-2; 5q-, del(5q); EB-1, excess blasts-1; sAML, secondary acute myeloid leukemia; HC, healthy control. Cells are color-coded according to samples. (B) UMAP of HSPC subclusters. Cells are color-coded according to the defined subset. (C) Heatmap displaying scaled expression of canonical cell type-associated genes for clusters. (D) Stacked bar plots show the percentage of sample contributions per annotated cell type (left) and the percentage of annotated cell type contributions per sample (right). (E) Boxplot showing the fraction of CMP and EBMP cluster in HCs (red) and MDS-EB/sAML patients (blue) samples. The p values were calculated using two-tailed Student’s t test; *p < 0.05