Fig. 6

Tregs did not correlate with response to ICIs, but with survival. A Histograms represent the percentage of total, active, resting, and non-suppressive Tregs in NSCLC patients under immunotherapy treatment as the first line. Black histograms showed the median levels of the different populations of Tregs in responder patients (R), and the gray histograms represented the median values of non-responders (NR). B Kaplan Meier curves of PFS and OS of total Tregs evaluated at baseline using as cut-off a value of 6.2%. Long-rank test was used to analyze the differences between the two groups. C Histogram represented the ratio of %total Treg/%CD137⁺ Tcells in responders (black histogram) and non-responders (gray histogram); Kaplan Meier curves of OS and PFS of %total Treg/%CD137⁺Tcells ratio evaluated at baseline using as cut-off a value of 5.5%. Long-rank test was used to analyze the differences between the two groups. D Histogram represented the ratio of %total Treg/%CD137⁺ T cells in responders (black histogram) and non-responders (gray histogram); Kaplan Meier curves of OS and PFS of %total Treg/%PMN(Lox1⁺)-MDSC ratio evaluated at baseline using as cut-off a value of 40%. Long-rank test was used to analyze the differences between the two groups. m = months. p values < 0.05 were considered significant