Fig. 5

Transferred DNT infiltrate skin tissue via CCR5. A Biological process analysis was performed on the basis of DEGs with significantly upregulated and downregulated expression in the skin of IMQ-induced murine psoriasis compared with control mice. B Heatmap showing genes related to C–C motif chemokine ligands in mouse skin from IMQ-treated and control mice. C mRNA levels of Ccl3, Ccl4, Ccl5, Ccl17, and Ccl22 in mouse skin from IMQ-treated and control mice. D Flowchart of Ccr5^−/− or WT DNT administration in IMQ-induced psoriasis. E Representative flow cytometry plots and statistical analysis of the percentages of transferred DNT (CD45.2⁺CD3⁺ cells in total single cells) that migrated to skin tissue, LNs and spleen. F Representative H&E staining of skin sections obtained from IMQ-induced psoriasis and WT or Ccr5^−/− DNT-treated mice. The scale bar represents 200 µm. G Statistical analysis of the layers of keratinocytes in HE sections obtained from IMQ-induced psoriasis and WT or Ccr5^−/− DNT-treated mice. H GSEA of the IL17A signaling pathway and keratinization in the skin from Ccr5^−/− vs. WT DNT-treated mice. I Heatmap showing genes related to cytokines and cytokine receptors in the skin from Ccr5^−/− or WT DNT-treated mice. J Statistical analysis of the percentages of γδ^low T cells in skin CD3⁺ T cells from Ccr5^−/− or WT DNT-treated mice. K Statistical analysis of the percentages of Zombie Aqua⁺ γδ^low T cells in the skin of Ccr5^−/− or WT DNT-treated mice. L Representative flow cytometry plots and statistical analysis of the percentages of IL17A⁺ cells in skin γδT cells from Ccr5^−/− or WT DNT-treated mice. Data are presented as the mean ± SD; n = 5 mice/group. The normal distribution of variables was assessed using the Shapiro‒Wilk test. One-way ANOVA with a post hoc test for normal variables and the Kruskal‒Wallis test for nonnormal variables were used for multiple comparisons. *p < 0.05, **p < 0.01