Fig. 4

DNT suppressed IL17A-producing γδ T cells through the NKG2D/NKG2DL axis. A Heatmap showing genes related to NKG2D ligands in γδ T cells from mouse skin DLNs. B and C Representative flow cytometry plots and statistical analysis of the percentages of MICAL1⁺ cells in γδ T cells from the skin, LNs and spleen. D and E Representative flow cytometry plots and statistical analysis of the percentages of MICAL2⁺ cells in γδ T cells from the skin, LNs and spleen. F Statistical analysis of the percentages of MICAL1⁺ cells in skin γδ^high T and γδ^low T cells. G Statistical analysis of the percentages of MICAL2⁺ cells in skin γδ^high T and γδ^low T cells. H and I Statistical analysis of the percentages of MICAL1/2⁺ in Rorc-tdTomato-positive or Rorc-tdTomato-negative γδ T cells from the skin, LNs and spleen. J and K Statistical analysis of the percentages of Rorc-tdTomato⁺MICAL1⁺ or Rorc-tdTomato⁺ MICAL2⁺ cells in skin γδ^high T and γδ^low T cells. L and M Statistical analysis of the percentages of Annexin V⁺ cells in MICAL1/2-positive or MICAL1/2-negative γδ T cells with or without DNT. N Statistical analysis of the percentages of cleaved caspase3⁺ cells in γδ T cells with or without NKG2D blocking antibody. O Statistical analysis of the percentages of Annexin V⁺ cells in γδ T cells with or without NKG2D blocking antibody. P Statistical analysis of the percentages of Zombie Aqua⁺ cells in γδ T cells with or without NKG2D blocking antibody. Q Statistical analysis of the percentages of IL17A⁺ cells in γδ T cells with or without NKG2D blocking antibody. R Statistical analysis of the percentages of cleaved caspase3⁺ cells in Rorc-tdTomato-positive or -negative γδ T cells with or without NKG2D blocking antibody. (S) Statistical analysis of the percentages of Annexin V⁺ cells in Rorc-tdTomato-positive or -negative γδ T cells with or without NKG2D blocking antibody. Data are presented as the mean ± SD; A, B n = 3 mice/group, C–J n ≥ 5 mice/group, K–S n ≥ 4 biologically independent samples per group. The normal distribution of variables was assessed using the Shapiro‒Wilk test. One-way ANOVA with a post hoc test for normal variables and the Kruskal‒Wallis test for nonnormal variables were used for multiple comparisons. *p < 0.05, **p < 0.01