Fig. 3
Transferred DNT suppressed the presence of IL17A-producing γδ T cells. A Representative flow cytometry plots and statistical analysis of the percentages of skin γδhigh T and γδlow T cells relative to the total number of γδ T cells, CD3+ T cells and CD45.2+ cells in each group. B Absolute numbers of γδhigh T and γδlow T cells in mouse skin. C Representative flow cytometry plots and statistical analysis of the percentages of γδT cells relative to the total number of CD3+ T cells and CD45.2+ cells in LNs and spleens. D Representative flow cytometry plots and statistical analysis of the percentage of IL-17A+ cells among skin, LN and spleen γδ T cells. E Representative flow cytometry plots and statistical analysis of the percentages of IL-17A+ cells among skin γδhigh T and γδlow T cells in each group. F Statistical analysis of IL17A+ cells among Rorc-tdTomato-positive and Rorc-tdTomato-negative γδT cells in the skin, LNs and spleen. G Representative flow cytometry plots and statistical analysis of IL17A and tdTomato were measured in skin γδhigh T and γδlow T cells by using flow cytometry. H, I Representative flow cytometry plots and statistical analysis of the percentages of IL17A and tdTomato in the skin, LN and spleen. J Representative flow cytometry plots and statistical analysis of the percentages of Zombie Aqua+ γδ T cells in the skin, LNs and spleen. K Representative flow cytometry plots and statistical analysis of the percentages of Zombie Aqua+ cells in skin γδhigh T and γδlow T cells. L Statistical analysis of the percentages of apoptotic and relative apoptotic γδT cells. M Statistical analysis of the percentages of relative apoptotic cells in Rorc-tdTomato-positive or Rorc-tdTomato-negative γδ T cells. Data are presented as the mean ± SD; A–I n = 6 mice/group, J–L n = 5 mice/group, M n = 4 biologically independent samples per group. The normal distribution of variables was assessed using the Shapiro‒Wilk test. One-way ANOVA with a post hoc test for normal variables and the Kruskal‒Wallis test for nonnormal variables were used for multiple comparisons. *p < 0.05, **p < 0.01