Fig. 2

Transcriptome sequencing analysis of skin and DLNs in IMQ-induced murine psoriasis with or without DNT treatment. A Distributions of differentially expressed genes with upregulated (274) and downregulated (206) expression in the skin of IMQ-induced murine psoriasis with or without DNT treatment are depicted in a volcano plot. (P value < 0.05 and an absolute fold change ≥ 1.5. n = 3/group). B and C Biological process and KEGG pathway analyses were performed on the basis of differentially expressed genes with significantly upregulated and downregulated expression in the skin of IMQ-induced murine psoriasis with or without DNT treatment. D Heatmap showing the genes associated with psoriasis development in the skin of IMQ-induced murine psoriasis, including the DNT treatment group, psoriasis group, and control group. E and F Heatmap displaying the genes related to the immune response and inflammatory response in the skin of IMQ-induced murine psoriasis, including DNT treatment, psoriasis, and control groups. G Heatmap showing genes related to the immune response and cytokine regulation in the lymph nodes of the DNT treatment, IMQ-induced psoriasis, and control groups. H GSEA of leukocyte activation involved in the inflammatory response, keratinization and chemokine signaling pathways in the skin from the DNT treatment and IMQ-induced psoriasis groups. I Relative mRNA level of IL17A in skin tissue. Data are presented as the mean ± SD; n = 5 mice/group. The normal distribution of variables was assessed using the Shapiro‒Wilk test. One-way ANOVA with a post hoc test for normal variables and the Kruskal‒Wallis test for nonnormal variables were used for multiple comparisons. *p < 0.05, **p < 0.01