Fig. 3
Identification of M2-like Ccl8high Macrophages in the LLC-bearing murine model. A UMAP plot showing the annotation of macrophage populations in LLC tumors. B Feature plot and C Dotplot showing marker genes of macrophage populations. D Cell proportion of each cell type in NT and RT groups. E Heatmap displaying scores of M1, M2, angiogenesis, phagocytosis for each macrophage population. F Correlation analysis of CCL8 and CD163 expression level in TCGA-LUAD datasets (Spearman’s rho value = 0.542, p < 0.001). G The Gene Ontology (GO) enrichment analysis of each macrophage populations. H Heatmap displaying transcription factors activity of the Mac_Ccl8, Mac_Hmox1, Mono_Cxcl3, and Mono_Plac8 populations. I Development trajectory of macrophages populations predicted by Monocle2. J The cell density and K gene expression patterns along with the pseudotime,