Fig. 5
THOC7-AS1 promotes cSCC progression and associates with OCT1-FSTL1. (A) RNA-seq for key lncRNAs. (B) THOC7-AS1 expression in tissues. (C) THOC7-AS1 expression in cell lines. (D) THOC7-AS1 FISH in A431, scale bars 100 μm. (E) THOC7-AS1 knockdown efficiency. (F) OCT1, ZEB1, and FSTL1 levels after THOC7-AS1 knockdown. (G–H) Clone formation after THOC7-AS1 knockdown, scale bars 1 cm. (I) Cell viability after THOC7-AS1 knockdown. (J–K) Migration by scratch assay, scale bars 100 μm. (L–M) Invasion by Transwell, scale bars 50 μm. (N) THOC7-AS1 and OCT1 binding prediction. (O–P) Silver staining and western blot after RNA pull-down. (Q) THOC7-AS1 RNA levels after RIP. (R) THOC7-AS1 and OCT1 FISH co-localization, scale bars 100 μm. Data were presented as the mean ± SD. (ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)