Fig. 5

Gene expression analysis quantified by real-time PCR (qPCR) in N + lung SCC samples. A-C parts show qRT-PCR analysis of ZNF334 and TINAGL1 gene expression in the different types of lung squamous cancer cell lines ((A): SK-MES-1, (B): NCI-H520 and (C): NCI-H2170, respectively) and normal human lung cell line (MRC-5). Part D, shows qRT-PCR analysis of ZNF334 and TINAGL1 gene expression in the lung SCC lymphatic metastatic tissues and lung non-tumor tissues. Gene expression levels were normalized to the housekeeping gene (GAPDH). Data are expressed using the 2^−∆∆Ct method. Non-parametric Wilcoxon test was carried out to evaluate statistical differences between groups. Differences were considered significant at p < 0.05