Fig. 8

LETM2 exerts pro-carcinogenic effects by activating the MTOR pathway. A Western Blot assay to detect changes in the MTOR pathway after overexpression of LETM2. B Western Blot assay to detect changes in the MTOR pathway after treatment with rapamycin. C ATP proliferation rate assay to detect the effect of treatment with rapamycin on AGS cell proliferation. D ATP proliferation rate assay to detect the effect of rapamycin treatment on the proliferation of SGC7901 cells. E soft agar assay to detect the effect of treatment with rapamycin on the anchorage-independent growth capacity of AGS cells. F soft agar assay to detect the effect of treatment with rapamycin on the anchorage-independent growth capacity of SGC7901 cells G plate cloning assay to detect the effect of treatment with rapamycin on the proliferation of AGS cells. H Plate cloning assay to detect the effect of treatment with rapamycin on the proliferation of SGC7901 cells