Fig. 6

LETM2 significantly promoted the proliferation and metastatic ability of gastric cancer cells. A Identification of stably transfected cells overexpressing exogenous LETM2. B ATP proliferation rate assay to detect the effect on AGS cell proliferation after overexpression of LETM2. C ATP proliferation rate assay to detect the effect of overexpression of LETM2 on the proliferation of SGC7901 cells. D, E soft agar assay to detect the effect on the anchorage-independent growth capacity of AGS、 SGC7901 cells after overexpression of LETM2. F, G Plate cloning assay to detect the proliferation of ability of AGS、SGC7901 cells after overexpression of LETM2. H Flow cytometry detection of AGS(Vector), AGS(LETM2) cell cycle distribution. I Flow cytometry detection of SGC7901(Vector), SGC7901(LETM2) cell cycle distribution. J Western Blot experiments were performed to detect the relevant cyclins that regulate the G0/G1 phase. K Subcutaneous transplanted tumors were photographed. L Tumors were weighed