Fig. 2

Knockout of Rab5a attenuates membrane TLR4 expression on macrophages and inhibits the activation of macrophages. BMDMs from wild-type and Rab5a^−/− C57BL mice were treated with LPS (1 μg/ml) for the indicated time. A Membrane TLR4 expression of BMDMs was analyzed by flow cytometry. B Proinflammatory factor mRNA levels in BMDMs were measured by qPCR. C Western blotting of MAPK levels in BMDMs. D Representative image of TLR4 (red) and GM130 (green) immunofluorescence staining in BMDMs, scale bars: 5 μm. Data are expressed as the mean ± SD, n = 5, *p < 0.05, **p < 0.01, ***p < 0.001, 1-way ANOVA with Tukey’s post hoc test