Fig. 2

Preparation and characterization of the chemoimmunotherapy hydrogel. A Pictures of hydrogel containing sodium alginate (ALG) solution (20 mg mL⁻¹), CaCl₂ (1.8 × 10^–3 mol L⁻¹) and Cy5 (0.1 mg mL⁻¹) formed in vitro at room temperature. B Pictures of hydrogels containing sodium ALG solution (20 mg mL⁻¹) formed in vivo. C Scanning electron microscope images of blank ALG hydrogel (the scale bar is 100 µm). D Near-infrared (NIR) images of tumor-bearing ICR mice were taken on 1 h, 4 h, 12 h, 48 h, 72 h and 120 h, after peritumoral injection with pure Cy5 dye, or ALG hydrogel loaded with Cy5 (n = 3). E Relative total radiant efficiency of ICR mice after peritumoral injection with pure Cy5 dye, or ALG hydrogel loaded with Cy5 (n = 3). The error bars represented mean ± SEM