Fig. 2

AURKB knockdown suppresses cell proliferation, cell cycle progression, migration and invasion, and upregulates cellular senescence. A The RT-qPCR analyses detected shRNAs transfection efficiency in T24 and 5637 cells. B Cell viability was measured by the CCK-8 assay in T24 and 5637 cells. C The colony-forming assay detected the proliferation ability of a single tumor cell. D IF staining for KI67 (red) and counter-staining nuclei with DAPI (blue) in T24 and 5637 cells. E Cell cycle analysis was measured by flow cytometry in T24 and 5637 cells. F Representative images of SA-β-gal staining for T24 and 5637 cells. G The wound healing assay demonstrated the capacity of migration in T24 and 5637 cells. H The transwell assay demonstrated the capacity of invasion in T24 and 5637 cells. (The magnification under the microscope is shown as marked in the figure. *p < 0.05, **p < 0.01)