Fig. 5

Notch inhibitors diminish the COMP-induced migration and tumorspheres size of ovarian cancer cells. Transwell migration assays for SKOV3 and OAW42 cells treated with COMP (COMP), DAPT (10 μM), and COMP in combination with DAPT (COMP + DAPT). A Representative images and B, C the number of migrated cells in the presence of DAPT. The number of migrated cells was normalized to the number of control cells (PBS-treated). D–F The same experiments were performed using an anti-Jagged1 antibody (5 μg/ml) in the presence or absence of COMP. In both experiments, 62.5 μg/ml and 12.5 μg/ml of COMP were used for SKOV3 and OAW42 cells treatment, respectively. PBS-treated cells were also used as a control. G–I Tumorsphere formation assays were performed for SKOV3 and OAW42 cells treated with COMP (20 μg/ml), DAPT (1 μM), and anti-Jagged1 antibody (2 μg/ml). PBS-treated cells were used as a control. A minimum of 10 spheroids per well were measured. Scale bar: 100 µm. The p-value was calculated by one-way ANOVA followed by Tukey’s post-test. The data are representative of at least three independent experiments, and graphs depict the mean with the standard deviation. P < 0.05 was considered statistically significant. (*, **, ***, ****, and ns indicate p < 0.05, p < 0.01, p < 0.001, p < 0.0001, and non-significant, respectively)