Fig. 1

PI16-deficiency in Treg promotes the differentiation and proliferation of Tregs. A Diagram for the construction of PI16 conditional knockout mice; B expression level of Foxp3 (n = 5, 35.12 ± 5.71% vs. 20.00 ± 1.61%, p = 0.034) in vitro Treg induction assay; C the proliferation level of iTreg (n = 3, 50.11 ± 12.33% vs. 11.47 ± 2.41%, p = 0.037); D, E PD-1 (D, n = 3, 50.11 ± 12.33% vs. 11.47 ± 2.41%, p = 0.005) and Helios expression (E, n = 5,17.4 ± 2.56% vs. 8.75 ± 1.40%, p = 0.018) in vitro Treg induction assay. *p < 0.05, **p < 0.01