Fig. 4
From: Intestinal Piezo1 aggravates intestinal barrier dysfunction during sepsis by mediating Ca2+ influx
Piezo1 Induces Mitochondrial Impairment in Caco-2 Cells In Vitro. A, B Measurement of mitochondrial ROS through MitoSOX, along with quantitative analysis of immunofluorescence evaluation (n = 3 for each group). Scale bar, 50 μm. C, D Assessment of mitochondrial membrane potential using JC-1 probes, with the relative intensity of red fluorescence to green fluorescence was used for quantitative analysis (n = 3 for each group). Scale bar, 50 μm. E–G Immunofluorescence analysis of ZO1 and Occludin in Caco-2 cell monolayers, accompanied by quantification of immunofluorescence analysis (n = 3 for each group). Scale bar, 20 μm