Fig. 4

Impact of GCs exposure on M28z and MBBz CAR + T cells phenotype and functions. Relative surface expression of LAG-3, PD-1 and TIM-3 in CAR + and UT fractions of CD4 + M28z and MBBz T cells following 3 exposures with Dx (A) or MP (B). C Representative histogram of M28z and MBBz CAR T cells proliferation after 3 exposures with or without Dx over 6 days. D Relative frequency of low proliferative cells in the CAR + and UT fractions of M28z and MBBz T cells. E Pie chart showing the number of functions of CD4 + and CD8 + M28z and MBBz CAR T cells without GC exposure compared to Dx or MP exposure following 3 stimulations with MSLN + K562 tumor cells. Relative frequency of CD107a + (F), TNF (G), IFNγ + (H) and IL-2 + (I) CD4 + and CD8 + M28z and MBBz CAR T cells without GC exposure compared to Dx or MP exposure after MSLN + K562 tumor cells stimulation A, B n = 11, D n = 3, F n = 5 and E n = 5 donors. Mann–Whitney test and Student t test were used to compare the functions in M28z vs. MBBz. Friedman test with Dunn’s correction was used to compare multifunction in No GC vs. Dx vs. MP conditions. Medians are represented. * p < 0.05, ** p < 0.01