Routine evaluation of HBV-specific T cell reactivity in chronic hepatitis B using a broad-spectrum T-cell epitope peptide library and ELISpot assay

Table 3 Stratification analyses of HBV-specific T cell reactivity for 203 CHB patients

	ELISpot (SFUs)	HBV DNA median (min–max)	n	HBsAg (IU/ml) median (min–max)	n	HBeAg (COI) median (min–max)	n	ALT (IU/L) median (min–max)	n
low	0–24	4.04 (0.3–7.04)	27	3075 (0.05–8895)	42	47.19 (0.079–158.5)	30	31.6 (11.4–391.2)	50
inter	25–90	2.69 (0.3–8.69)	58	1474.5 (0.05–52000)	91	3.7 (0.073–1726)	64	28.7 (10.2–243.4)	99
high	91–622	2.69 (0.3–5.99)	24	652.8 (0.05–20428)	45	0.336 (0.072–63.96)	30	28 (8.2–105.6)	51
Statistics
P^a		p = 0.0078		p < 0.0001		p = 0.00025		p = 0.048
P^b	Low versus inter	p = 0.015		p = 0.049				p = 0.022
	Low versus high	p = 0.005		p = 0.0053		p = 0.00018		p = 0.04
	Inter versus high			p = 0.0053		p = 0.00017
P^c		p = 0.34638		p = 0.00723				p = 0.16839

HBV-DNA (reference values: < 2.69 lg IU/mL), HBsAg (reference values: 0–0.05 IU/mL), HBeAg (reference values: 0–1 COI; COI, cut off index, COI = sample value/cut off value), ALT (reference values: < 40 IU/L)
P^a, Kruskal–Wallis test (K–W); P^b, Mann–Whitney test (M–W); P^c, Multivariate linear regression analysis

ISSN: 1479-5876