Fig. 2

MiRNA-21a-5p treatment enhances inflammatory cell infiltration into the skin of bleomycin mice. On days 14, 21, and 28 from the start of bleomycin injection, plasmid containing of 100 μg pre-miRNA-21a-5p (n = 5) or pre-miR-NC as a control (n = 5) in 1 mL saline was hydrodynamically injected into the mice. The mice were sacrificed on day 33 from the start of bleomycin injection, and immunohistochemistry was performed on the skin tissue. Sections of skin tissue were stained with antibodies against TNF-α, IL-β, IL-6, and IL-17. The graphs present the number of antibody-positive cells (mean ± SD, n = 5/group). Original magnification: 200 × . Scale bar: 100 µm