Fig. 8
IDO1high OC cell-derived EVs activated endothelial mitophagy by upregulating NAD + level and sirt3 expression. a Level of L-kyn in cell supernatant and cell derived-EVs of Ctrl EVs,IDO1-OE EVs or IDO1-sh EVs (n = 5, Mean ± SD, two-way ANOVA, ***P < 0.001). b Level of L-kyn in the endothelial cells incubated with different groups (n = 5, Mean ± SD, two-way ANOVA, ***P < 0.001, ns. no significance). c Level of NAD + /NADH in the endothelial cells treated with Ctrl EVs, IDO1-OE EVs or IDO1-sh EVs (n = 5, Mean ± SD, one-way ANOVA, *P < 0.05, ***P < 0.001). d Representative images and quantification analysis of sirt3 protein expression in the endothelial cells incubated with Ctrl EVs, IDO1-OE EVs or IDO1-sh EVs by Western blot (n = 3, Mean ± SD, one-way ANOVA, * P < 0.05,**P < 0.01). e Representative images and quantification analysis of IDO1 protein expression in the endothelial cells incubated with Ctrl EVs, IDO1-OE EVs or IDO1-sh EVs by Western blot (n = 3, Mean ± SD, one-way ANOVA, ***P < 0.001). f Co-immunoprecipitation (Co-IP) of sirt3 and sirt3-acetylation in endothelial cells after treatment with Ctrl EVs, IDO1-OE EVs or IDO1-sh EVs