Fig. 7
IDO1high OC cell-derived EVs facilitated tumor angiogenesis by triggering PINK1-Parkin-mediated mitophagy of endothelial cells. a Intracellular ATP levels of the endothelial cells incubated with ctrl EV, IDO1-OE EV or IDO1-sh EV (n = 5, Mean ± SD, one-way ANOVA, *P < 0.05,**P < 0.01,***P < 0.001). b MitROS production of the endothelial cells incubated with ctrl EV, IDO1-OE EV or IDO1-sh EV (n = 5, Mean ± SD, one-way ANOVA, *P < 0.05,**P < 0.01,***P < 0.001). c Mitochondria membrane potential of the endothelial cells incubated with CCCP, ctrl EV, IDO1-OE EV or IDO1-sh EV. Scale bar:10 μm. (n = 5, Mean ± SD, one-way ANOVA, *P < 0.05, ***P < 0.001). d Representative images and quantification of LC3II/LC3I ration and IDO1 protein expression in the endothelial cells treated with ctrl EV, IDO1-OE EV or IDO1-sh EV by Western blot (n = 3, Mean ± SD, one-way ANOVA, *P < 0.05, ***P < 0.001). e Representative images and qualification of PINK1, Parkin protein expression in the mitochondria isolated from the endothelial cells treated with ctrl EV, IDO1-OE EV or IDO1-sh EV by Western blot (n = 3, Mean ± SD, one-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001). f Representative images and qualification of IDO1 protein expression in the mitochondria isolated from the endothelial cells treated with ctrl EV, IDO1-OE EV or IDO1-sh EV by Western blot (n = 3, Mean ± SD, one-way ANOVA, ***P < 0.001). g Representative images and quantitative analysis of tube formation in the different treated endothelial cells. Scale bar:500 μm. (n = 5, Mean ± SD, one-way ANOVA, ***P < 0.001, ns. no significance). h Representative images and quantitative analysis of wound healing assay for the endothelial cells in different groups. Scale bar:500 μm. (n = 5, Mean ± SD, one-way ANOVA, ***P < 0.001, ns. no significance)