Fig. 6
IDO1high OC cell-derived EVs promoted tumor angiogenesis and development. a Representative images and quantitative analysis of tube formation assay for endothelial cells incubated with negative control (Negative ctrl), IDO1-control A2780 EVs (ctrl EV),IDO1-overexpression A2780 EVs(IDO1-OE EV) or IDO1-knockdown A2780 EVs(IDO1-sh EV).Scale bar:500 μm. (n = 5, Mean ± SD, one-way ANOVA, ***P < 0.001). b Representative images and quantitative analysis of wound healing assay for endothelial cells treated with Negative ctrl, ctrl EV, IDO1-OE EV or IDO1-sh EV. Scale bar:500 μm. (n = 5, Mean ± SD, one-way ANOVA, **P < 0.01, ***P < 0.001). c Representative images of the primary ovary tumor size in orthotopic OC model education with Ctrl EV or IDO1-OE EV. bar = 1 cm. (n = 5, Mean ± SD, t-test, ***P < 0.001). d Numbers of peritoneal metastases validated by HE staining in orthotopic OC model education with Ctrl EV or IDO1-OE EV. Scale bar:50 μm. (n = 5, Mean ± SD, t-test, **P < 0.01). e IHC analysis of CD31 expression in primary tumor tissues of the orthotopic OC model education with Ctrl EV or IDO1-OE EV. Scale bar:50 μm. (n = 3, Mean ± SD, t-test, **P < 0.01). f Representative images of LC3, TOMM20and CD31 in primary tumor tissue sections of orthotopic OC model educated with Ctrl EV or IDO1-OE EV by immunofluorescence staining. Scale bar:20 μm