Fig. 5
Aberrant IDO1 upregulation involved in tryptophan metabolic reprogramming of OC cells. a Dot plot showing IDO1 expression of OC and non-OC samples. b Visualization of IDO1 expression in major cell clusters of OC and non-OC ovarian samples. c Visualization of IDO1 expression in epithelial cell subclusters with OC and non-OC ovarian samples. d Heatmap showing IDO1 expression in OC samples from OCGA_OV dataset and non-OC samples from GETx dataset. e Heatmap showing IDO1 expression in OC samples from GSE140082 dataset and non-OC samples from GETx dataset. f Immunohistochemistry analysis of IDO1 protein expression between OC and non-OC ovarian tissues from the human protein atlas (HPA)database. Scale bar:200 μm. g Immunohistochemistry detecting of IDO1 protein expression in tissue microarray composed of OC (n = 115) and non-OC (n = 80) ovarian tissues from our hospital. Scale bar:200 μm. (u-test, ***P < 0.001). h IDO1 mRNA expression between non-OC cells (IOSE80) and OC cells (A2780, SKOV3) was detected by qRT-PCR (n = 3, Mean ± SD, t-test, **P < 0.01, ***P < 0.001). i IDO1 protein expression between non-OC cells (IOSE80) and OC cells (A2780, SKOV3) was detected by Western blot (n = 3, Mean ± SD, t-test, ***P < 0.001). For a–c, all n = 7 OC and n = 5 non-OC samples obtained from GSE184880 dataset were used for analysis