Fig. 2

OC cell-derived EVs promoted angiogenesis by activating endothelial mitophagy in vitro. a Representative images of Mitotracker, LC3 and DAPI in endothelial cells incubated with different groups imaged by confocal microscopy. Scale bar: 10 μm. b LC3⁺/Mitotracker⁺ fluorescence intensity ratio in the different treated endothelial cells (n = 5, Mean ± SD, one-way ANOVA, ***P < 0.001). c Representative images of PINK1, Parkin, LC3 and Tublin protein expression in the different incubated endothelial cells by Western blot. d–f Quantification of PINK1, Parkin expression and LC3II/LC3I ratio in the different incubated endothelial cells by Western blot (n = 3, Mean ± SD, one-way ANOVA, ** P < 0.01,***P < 0.001). g Representative images and quantitative analysis of tube formation in different groups. Scale bar:500 μm. (n = 5, Mean ± SD, one-way ANOVA, ***P < 0.001)