Fig. 6

Downregulation of NRK inhibited cell proliferation, induced cell apoptosis, arrest cell cycle and suppressed migration. A The typical graph of EdU staining of si-control and si-NRKs groups. Nuclei were stained by DAPI (blue). B CCK-8 assay was used to detect the viability of the WPMY-1 cells treated by si-control (black line) and si-NRKs (red line and yellow line). C Flow cytometry analysis of alterations of cells apoptosis in WPMY-1 cells transfected with si-control and si-NRKs. PI PE-A in y-axis stands for the fluorescence intensity of propidine iodide (PI) and FITC-A in x-axis stands for the fluorescence intensity of Fluorescein isothiocyanate (FITC) laballed Annexin V. Calculation area of the apoptosis rate was percentage of Annexin V + /PI + cells. D Statistical analysis of cell apoptosis rate according to flow cytometry analysis. E Flow cytometry analysis for proportion of three cell cycle phase. F Statistical analysis of cell cycle stages according to flow cytometry analysis. G Wound healing assay of WPMY-1 cells with or without NRK silencing in 24 and 48 h. H Statistical analysis of relative migration rate according to wound healing assay. I Transwell assay of WPMY-1 cells with or without NRK silencing. J Statistical analysis of migration cells according to transwell assay. All values shown are mean ± SD of triplicate measurements and repeated three times with similar results, *Means p < 0.05 and **means p < 0.01