Fig. 7

WIF1 alleviates HG-induced glycolytic dysfunction in 661 W cells and protects photoreceptor cells. A Western blot and quantification of HK2, PKM2 (C), PFKFB3, PDK1, WIF1, and LDHA protein levels in 661 W cells. B-C RT-qPCR analysis of the mRNA levels of the genes encoding glycolysis enzymes in 661 W cells. D Levels of secreted lactate of 661 W cells of four groups. E, F Proliferation detection via the CCK-8 assay. Results are expressed as OD values (450 nm). G Flow cytometric analysis was employed to detect the ROS of the four 661 W cell groups, followed by an assessment of mean fluorescence intensity (MFI). The presented data depict the average and standard deviation from a minimum of three repetitions, with differences assessed using standard one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001