Fig. 2

WIF1 overexpression has a positive protective effect on photoreceptor injury in db/db diabetic mice. A Representative image of retinal OCT in four groups of mice. Quantification of RNFL, GCL + OPL, total retinal thickness, and outer retinal thickness is shown within the circular region adjacent to the optic nerve head. n = 6 B Representative retinal H&E staining images for the four groups. Quantitative analysis of total retinal thickness within the circular area surrounding the optic nerve head and photoreceptor cell nuclei in the retinal ONL. Scale bar, 50 μm. n = 6 C Representative images depicting TUNEL (red) staining in retinal sections from the four groups of mice. The relative fluorescence ratio of TUNEL was subsequently analyzed. The presented data depict the average and standard deviation from a minimum of three repetitions, with differences assessed using standard one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001