Fig. 3

Modulation of DEGs in GBM cells by guadecitabine treatment. A Pie of pie charts showed number and percentage of genes belonging to clusters #1, #2, and #6 (see Fig. 1) that were significantly (p-value < 0.05) up-regulated (FC > 1) or down-regulated (FC < 1) in DHA-treated vs untreated GBM cell lines (expression), and number and percentage of up-regulated genes with ipo- (βvalue < 0) or iper- (βvalue > 0) methylated promoter regions after DHA treatment (methylation, FC βvalue). N/A, genes with no information on the methylation level of their promoter. B Enrichment analyses of genes up-regulated by DHA were performed by EnrichR tool. X axis reports the Combined Score of significant enriched GO terms. Size of the dot represents the significance of GO terms; colour of the dot represents the different clusters identified by the clustering technique depicted in Fig. 1